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Literature summary extracted from

  • Mukerji, S.K.
    Corn leaf phosphoenolpyruvate carboxylases. Purification and properties of two isoenzymes (1977), Arch. Biochem. Biophys., 182, 343-351.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.31 0.047
-
phosphoenolpyruvate isoenzyme PC-II Zea mays
4.1.1.31 0.1
-
phosphoenolpyruvate isoenzyme PC-I Zea mays
4.1.1.31 1.28
-
HCO3- isoenzyme PC-II Zea mays
4.1.1.31 1.45
-
HCO3- isoenzyme PC-I Zea mays

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.1.31 Mg2+ activates Zea mays
4.1.1.31 Mg2+ Km for isoenzyme PC-II: 0.015 mM Zea mays
4.1.1.31 Mg2+ Km for isoenzyme PC-I: 0.032 mM Zea mays
4.1.1.31 Mn2+ Km for isoenzyme PC-II: 0.0067 mM Zea mays
4.1.1.31 Mn2+ Km for isoenzyme PC-I: 0.0102 mM Zea mays

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.31 225700
-
sucrose density gradient centrifugation, isoenzyme PC-I Zea mays
4.1.1.31 270800
-
sucrose density gradient centrifugation, isoenzyme PC-II Zea mays

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.31 Zea mays
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.31 isoenzyme PC-I and PC-II Zea mays

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.1.1.31 leaf
-
Zea mays
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.31 phosphoenolpyruvate + CO2
-
Zea mays phosphate + oxaloacetate
-
?
4.1.1.31 phosphoenolpyruvate + HCO3-
-
Zea mays phosphate + oxaloacetate
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.1.31 40
-
isoenzyme PC-I Zea mays

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.1.1.31 45
-
50% inactivation after 87 s, isoenzyme PC-I. 50% inactivation after 100 s, isoenzyme PC-II Zea mays
4.1.1.31 50
-
50% inactivation after 18 s, isoenzyme PC-I. 50% inactivation after 28 s, isoenzyme PC-II Zea mays
4.1.1.31 55
-
50% inactivation after 13 s, isoenzyme PC-I. 50% inactivation after 17 s, isoenzyme PC-II Zea mays