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Literature summary extracted from

  • Goto, Y.; Shimizu, J.; Shukuya, R.
    Purification and molecular characterization of mitochondrial phosphoenolpyruvate carboxykinase from bullfrog (Rana catesbeiana) liver (1980), J. Biochem., 88, 1239-1249.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.32 GMP competitive with GDP Lithobates catesbeianus
4.1.1.32 iodoacetamide guanine nucleotides, IDP and Mn2+ protect Lithobates catesbeianus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.1.1.32 mitochondrion
-
Lithobates catesbeianus 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.1.32 Mn2+ absolutely required for phosphoenolpyruvate carboxylation Lithobates catesbeianus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.32 65000
-
gel filtration Lithobates catesbeianus
4.1.1.32 70000
-
1 * 70000, SDS-PAGE Lithobates catesbeianus
4.1.1.32 71000
-
glycerol density gradient centrifugation Lithobates catesbeianus

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.32 Lithobates catesbeianus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.1.1.32 liver
-
Lithobates catesbeianus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.1.32 17.7
-
-
Lithobates catesbeianus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.32 GTP + oxaloacetate
-
Lithobates catesbeianus GDP + phosphoenolpyruvate + CO2
-
?
4.1.1.32 additional information ADP is completely inactive in carboxylation of oxaloacetate Lithobates catesbeianus ?
-
?
4.1.1.32 UDP + phosphoenolpyruvate + CO2 very low activity Lithobates catesbeianus UTP + oxaloacetate
-
?

Subunits

EC Number Subunits Comment Organism
4.1.1.32 monomer 1 * 70000, SDS-PAGE Lithobates catesbeianus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.32 6.2
-
phosphoenolpyruvate carboxylation and exchange reaction in presence of Mn2+ Lithobates catesbeianus
4.1.1.32 7.5
-
oxaloacetate decarboxylation in presence of Mn2+ Lithobates catesbeianus
4.1.1.32 7.5
-
oxaloacetate decarboxylation and exchange reaction in presence of Mg2+ Lithobates catesbeianus