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Literature summary extracted from

  • Hebda, C.A.; Nowak, T.
    The purification, characterization, and activation of phosphoenolpyruvate carboxykinase from chicken liver mitochondria (1982), J. Biol. Chem., 257, 5503-5514.
    View publication on PubMed

General Stability

EC Number General Stability Organism
4.1.1.32 2-mercaptoethanol facilitates loss of activity Gallus gallus
4.1.1.32 dithiothreitol stabilizes Gallus gallus

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.32 (Z)-Phosphoenol-3-bromopyruvate linear competitive Gallus gallus
4.1.1.32 2-oxoglutarate above 5.0 mM, weak Gallus gallus
4.1.1.32 alpha-(Dihydroxyphosphinylmethyl) acrylic acid weak linear competitive Gallus gallus
4.1.1.32 citrate above 5.0 mM, weak Gallus gallus
4.1.1.32 DL-isocitrate above 5.0 mM, weak Gallus gallus
4.1.1.32 malate above 5.0 mM, weak Gallus gallus
4.1.1.32 oxalate above 5.0 mM, weak Gallus gallus
4.1.1.32 succinate above 5.0 mM, weak Gallus gallus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.32 0.003
-
 GDP
-
 Gallus gallus
4.1.1.32 0.013
-
 IDP
-
 Gallus gallus
4.1.1.32 0.03
-
 (Z)-3-fluorophosphoenolpyruvate
-
 Gallus gallus
4.1.1.32 0.042
-
 GDP GDP in form of MnGDP- Gallus gallus
4.1.1.32 0.067
-
 phosphoenolpyruvate
-
 Gallus gallus
4.1.1.32 0.13
-
 ITP
-
 Gallus gallus
4.1.1.32 0.197
-
 IDP IDP in form of MnIDP- Gallus gallus
4.1.1.32 19
-
 CO2 CO2 in form of HCO3- Gallus gallus
4.1.1.32 40
-
 ITP ITP in form of MnITP2- Gallus gallus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.1.1.32 mitochondrion
-
 Gallus gallus 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.1.32 Mn2+ activates Gallus gallus
4.1.1.32 additional information no requirement for a monovalent cation Gallus gallus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.32 70500
-
 1 * 70500, SDS-PAGE under reducing conditions Gallus gallus
4.1.1.32 75400
-
 high speed equilibrium sedimentation Gallus gallus

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.32 Gallus gallus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.1.1.32 liver
-
 Gallus gallus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.32 GDP + (Z)-3-fluorophosphoenolpyruvate + CO2
-
 Gallus gallus ?
-
 ?
4.1.1.32 GDP + phosphoenolpyruvate + CO2
-
 Gallus gallus GTP + oxaloacetate
-
 r
4.1.1.32 GTP + oxaloacetate
-
 Gallus gallus GDP + phosphoenolpyruvate + CO2
-
 ?
4.1.1.32 IDP + phosphoenolpyruvate + CO2
-
 Gallus gallus ITP + oxaloacetate
-
 r
4.1.1.32 ITP + oxaloacetate
-
 Gallus gallus IDP + phosphoenolpyruvate + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.32 monomer 1 * 70500, SDS-PAGE under reducing conditions Gallus gallus