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Literature summary extracted from

  • Sabo, D.J.; Orlando, J.A.
    Isolation, purification, and some properties of reduced nicotinamide adenine dinucleotide phosphate-cytochrome C2 reductase from Rhodopseudomonas spheroides (1968), J. Biol. Chem., 243, 3742-3749.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.6.2.5 activity is lost overnight if the enzyme is diluted Cereibacter sphaeroides

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.6.2.5 iodoacetate
-
 Cereibacter sphaeroides
1.6.2.5 N-ethylmaleimide
-
 Cereibacter sphaeroides
1.6.2.5 NADH competitive inhibitor Cereibacter sphaeroides
1.6.2.5 p-chloromercuribenzoate
-
 Cereibacter sphaeroides
1.6.2.5 thyroxine
-
 Cereibacter sphaeroides

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.6.2.5 0.0125
-
 2,6-dichloroindophenol
-
 Cereibacter sphaeroides
1.6.2.5 0.037
-
 ferricytochrome c2
-
 Cereibacter sphaeroides
1.6.2.5 0.125
-
 ferricytochrome c2
-
 Cereibacter sphaeroides

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.6.2.5 43000
-
 gel filtration Cereibacter sphaeroides

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.6.2.5 2 ferricytochrome c2 + NADPH Rhodopseudomonas palustris
-
 2 ferrocytochrome c2 + NADP+ + H+
-
 ?
1.6.2.5 2 ferricytochrome c2 + NADPH Cereibacter sphaeroides native cytochrome is the natural physiological electron acceptor 2 ferrocytochrome c2 + NADP+ + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.6.2.5 Cereibacter sphaeroides
-
-
-
1.6.2.5 Rhodopseudomonas palustris
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.6.2.5
-
 Cereibacter sphaeroides

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.6.2.5 164
-
-
 Cereibacter sphaeroides

Storage Stability

EC Number Storage Stability Organism
1.6.2.5 -15°C, can be stored as lyophilized powder for at least 3 months Cereibacter sphaeroides
1.6.2.5 4°C, 0.4 M Tris-HCl, pH 8.0 purified concentrated enzyme is stable for approximately 2 weeks, gradual loss in activity can be restored by addition of glutathione to a final concentration of 0.0001 M, partially purified enzyme is stable for approximately 1 month Cereibacter sphaeroides

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.6.2.5 2 ferricytochrome c2 + NADPH
-
 Rhodopseudomonas palustris 2 ferrocytochrome c2 + NADP+ + H+
-
 ?
1.6.2.5 2 ferricytochrome c2 + NADPH contains additional NADP+ diaphorase activity Cereibacter sphaeroides 2 ferrocytochrome c2 + NADP+ + H+
-
 ?
1.6.2.5 2 ferricytochrome c2 + NADPH contains additional NADP+ diaphorase activity Rhodopseudomonas palustris 2 ferrocytochrome c2 + NADP+ + H+
-
 ?
1.6.2.5 2 ferricytochrome c2 + NADPH native cytochrome is the natural physiological electron acceptor Cereibacter sphaeroides 2 ferrocytochrome c2 + NADP+ + H+
-
 ?
1.6.2.5 2,6-dichloroindophenol + NADPH
-
 Cereibacter sphaeroides ? + NADP+
-
 ?
1.6.2.5 Fe(CN)6 3- + NADPH
-
 Cereibacter sphaeroides Fe(CN)6 4- + NADP+
-
 ?
1.6.2.5 horse heart cytochrome c + NADPH
-
 Cereibacter sphaeroides ? + NADP+
-
 ?
1.6.2.5 mammalian cytochrome c + NADPH
-
 Cereibacter sphaeroides ? + NADP+
-
 ?
1.6.2.5 additional information cytochrome c-553 is ineffective as an electron acceptor Cereibacter sphaeroides ?
-
 ?
1.6.2.5 NADPH + H+ + ferricytochrome c2
-
 Cereibacter sphaeroides NADP+ + ferrocytochrome c2
-
 r
1.6.2.5 Rhodospirillum rubrum cytochrome c2 + NADPH
-
 Cereibacter sphaeroides ? + NADP+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.6.2.5 NADPH-cytochrome f reductase
-
 Cereibacter sphaeroides

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.6.2.5 7.5
-
-
 Cereibacter sphaeroides

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.6.2.5 5.6 8
-
 Cereibacter sphaeroides

Cofactor

EC Number Cofactor Comment Organism Structure
1.6.2.5 FAD nonmetalloflavoprotein with FAD as prosthetic group Cereibacter sphaeroides

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.6.2.5 0.055
-
 NADH
-
 Cereibacter sphaeroides