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Literature summary extracted from

  • Subramanian, V.; Liu, T.N.; Yeh, W.K.; Narro, M.; Gibson, D.T.
    Purification and properties of NADH-ferredoxinTOL reductase. A component of toluene dioxygenase from Pseudomonas putida (1981), J. Biol. Chem., 256, 2723-2730.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.18.1.3 1,10-phenanthroline
-
 Pseudomonas putida
1.18.1.3 2,2'-dipyridyl
-
 Pseudomonas putida
1.18.1.3 PCMB
-
 Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.12.11 additional information
-
 additional information Km-values of the NADH-ferredoxinTOL reductase component: 0.0046 mM for cytochrome and 0.0105 mM for NADH Pseudomonas putida
1.18.1.3 0.0046
-
 ferricytochrome c
-
 Pseudomonas putida
1.18.1.3 0.0105
-
 NADH reaction with cytochrome c Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.12.11 46000
-
 1 * 46000, NADH-ferredoxinTOL reductase component, SDS-PAGE Pseudomonas putida
1.14.12.11 46500
-
 NADH-ferredoxinTOL reductase component, gel filtration Pseudomonas putida
1.18.1.3 46000
-
 1 * 46000, SDS-PAGE Pseudomonas putida
1.18.1.3 46500
-
 gel filtration Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
1.14.12.11 Pseudomonas putida
-
-
-
1.18.1.3 Pseudomonas putida
-
 NAD-ferredocinTOL reductase component of toluene dioxygenase
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.12.11 NADH-ferredoxinTOL reductase component of toluene dioxygenase Pseudomonas putida
1.18.1.3
-
 Pseudomonas putida

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.18.1.3 12.3
-
-
 Pseudomonas putida

Storage Stability

EC Number Storage Stability Organism
1.14.12.11 -20°C, purified NADH-ferredoxinTOL reductase component is stable for two weeks Pseudomonas putida
1.14.12.11 0-4°C, purified NADH-ferredoxinTOL reductase component is stable for up to 30 h Pseudomonas putida
1.18.1.3 -20°C, 3 months, little loss of activity Pseudomonas putida
1.18.1.3 0-4°C, stable for up to 30 h Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.12.11 additional information the NADH-ferredoxinTOL reductase component catalyzes the NADH-dependent reduction of 2,6-dichloroindophenol, nitroblue tetrazolium and ferricyanide. NADPH is inactive Pseudomonas putida ?
-
 ?
1.14.12.11 toluene + NADH + O2
-
 Pseudomonas putida (1S,2R)-3-methylcyclohexa-3,5-diene-1,2-diol + NAD+
-
 ?
1.18.1.3 2 ferricyanide + NADH
-
 Pseudomonas putida 2 ferrocyanide + NAD+ + H+
-
 ?
1.18.1.3 2 ferricytochrome c + NADH
-
 Pseudomonas putida 2 ferrocytochrome c + NAD+ + H+
-
 ?
1.18.1.3 ferricytochrome c + NADH
-
 Pseudomonas putida ferrocytochrome c + NAD+ + H+
-
 r
1.18.1.3 NADH + oxidized 2,6-dichlorophenolindophenol + H+
-
 Pseudomonas putida NAD+ + reduced 2,6-dichlorophenolindophenol
-
 ?
1.18.1.3 NADH + oxidized nitro blue tetrazolium + H+
-
 Pseudomonas putida NAD+ + reduced nitro blue tetrazolium
-
 ?
1.18.1.3 oxidized ferredoxin + NADH
-
 Pseudomonas putida reduced ferredoxin + NAD+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.12.11 monomer 1 * 46000, NADH-ferredoxinTOL reductase component, SDS-PAGE Pseudomonas putida
1.18.1.3 monomer 1 * 46000, SDS-PAGE Pseudomonas putida

Synonyms

EC Number Synonyms Comment Organism
1.18.1.3 NAD-ferredocinTOL reductase component of toluene dioxygenase Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.12.11 6.6 7.5 NADH-ferredoxinTOL reductase component Pseudomonas putida
1.18.1.3 6.6 7.5
-
 Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.12.11 FAD NADH-ferredoxinTOL reductase component is a flavoprotein that contains one mol of FAD per mol of enzyme. Km: 2.5 nM Pseudomonas putida
1.14.12.11 NADH NADH-ferredoxinTOL reductase component Pseudomonas putida
1.14.12.11 NADPH NADPH is inactive Pseudomonas putida
1.18.1.3 FAD can bind one mol of FAD per mol of enzyme, Km: 2.5 nM Pseudomonas putida
1.18.1.3 NADH
-
 Pseudomonas putida