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Literature summary extracted from

  • Jordan, A.; Torrents, E.; Jeanthon, C.; Eliasson, R.; Hellman, U.; Wernstedt, C.; Barbe, J.; Gibert, I.; Reichard, P.
    B12-dependent ribonucleotide reductases from deeply rooted eubacteria are structurally related to the aerobic enzyme from Escherichia coli (1997), Proc. Natl. Acad. Sci. USA, 94, 13487-13492.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.17.4.2 ATP
-
 Deinococcus radiodurans
1.17.4.2 ATP stimulates CDP reduction Thermotoga maritima
1.17.4.2 dATP
-
 Thermotoga maritima
1.17.4.2 dATP
-
 Lactobacillus leichmannii
1.17.4.2 dATP
-
 Deinococcus radiodurans

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.17.4.2 cloned by PCR from peptide sequence information Deinococcus radiodurans
1.17.4.2 cloned by PCR from peptide sequence information, nrdj gene sequenced completely and expressed in Escherichia coli BL21(DE3) Thermotoga maritima

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.17.4.2 dGTP inhibits CDP reduction Deinococcus radiodurans
1.17.4.2 dGTP inhibits CDP reduction Thermotoga maritima
1.17.4.2 dTTP inhibits CDP reduction Deinococcus radiodurans
1.17.4.2 dTTP
-
 Lactobacillus leichmannii
1.17.4.2 dTTP inhibits CDP reduction Thermotoga maritima

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.17.4.2 Ca2+
-
 Thermotoga maritima
1.17.4.2 Ca2+
-
 Deinococcus radiodurans
1.17.4.2 Mg2+
-
 Escherichia coli
1.17.4.2 Mg2+
-
 Thermotoga maritima
1.17.4.2 Mg2+
-
 Deinococcus radiodurans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.17.4.2 90000
-
-
 Pyrococcus furiosus
1.17.4.2 90000
-
 gel filtraton Thermotoga maritima
1.17.4.2 107000
-
 deduced amino acid sequence Deinococcus radiodurans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin + H2O Escherichia coli
-
 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin + H2O Pyrococcus furiosus
-
 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin + H2O Thermoplasma acidophilum
-
 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin + H2O Mycobacterium tuberculosis
-
 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin + H2O Methanocaldococcus jannaschii
-
 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin + H2O Archaeoglobus fulgidus
-
 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin + H2O Thermotoga maritima
-
 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin + H2O Lactobacillus leichmannii
-
 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin + H2O Chloroflexus aurantiacus
-
 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin + H2O Deinococcus radiodurans
-
 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin + H2O Deinococcus radiodurans R1 / ATCC 13939 / DSM 20539
-
 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin + H2O Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589
-
 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.17.4.2 Archaeoglobus fulgidus
-
-
-
1.17.4.2 Chloroflexus aurantiacus
-
 J-10-fl (ATCC 29366)
-
1.17.4.2 Deinococcus radiodurans
-
-
-
1.17.4.2 Deinococcus radiodurans R1 / ATCC 13939 / DSM 20539
-
-
-
1.17.4.2 Escherichia coli
-
-
-
1.17.4.2 Lactobacillus leichmannii
-
-
-
1.17.4.2 Methanocaldococcus jannaschii
-
-
-
1.17.4.2 Mycobacterium tuberculosis
-
-
-
1.17.4.2 Pyrococcus furiosus
-
-
-
1.17.4.2 Thermoplasma acidophilum
-
-
-
1.17.4.2 Thermotoga maritima
-
-
-
1.17.4.2 Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.17.4.2
-
 Thermotoga maritima
1.17.4.2
-
 Deinococcus radiodurans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.4.2 CDP + reduced thioredoxin
-
 Escherichia coli dCDP + oxidized thioredoxin + H2O
-
 ?
1.17.4.2 CDP + reduced thioredoxin
-
 Pyrococcus furiosus dCDP + oxidized thioredoxin + H2O
-
 ?
1.17.4.2 CDP + reduced thioredoxin
-
 Thermotoga maritima dCDP + oxidized thioredoxin + H2O
-
 ?
1.17.4.2 CDP + reduced thioredoxin
-
 Lactobacillus leichmannii dCDP + oxidized thioredoxin + H2O
-
 ?
1.17.4.2 CDP + reduced thioredoxin
-
 Deinococcus radiodurans dCDP + oxidized thioredoxin + H2O
-
 ?
1.17.4.2 CDP + reduced thioredoxin
-
 Deinococcus radiodurans R1 / ATCC 13939 / DSM 20539 dCDP + oxidized thioredoxin + H2O
-
 ?
1.17.4.2 CDP + reduced thioredoxin
-
 Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 dCDP + oxidized thioredoxin + H2O
-
 ?
1.17.4.2 CTP + reduced thioredoxin
-
 Thermotoga maritima dCTP + oxidized thioredoxin + H2O
-
 ?
1.17.4.2 CTP + reduced thioredoxin
-
 Lactobacillus leichmannii dCTP + oxidized thioredoxin + H2O
-
 ?
1.17.4.2 CTP + reduced thioredoxin
-
 Deinococcus radiodurans dCTP + oxidized thioredoxin + H2O
-
 ?
1.17.4.2 CTP + reduced thioredoxin
-
 Deinococcus radiodurans R1 / ATCC 13939 / DSM 20539 dCTP + oxidized thioredoxin + H2O
-
 ?
1.17.4.2 CTP + reduced thioredoxin
-
 Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 dCTP + oxidized thioredoxin + H2O
-
 ?
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin
-
 Escherichia coli 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin
-
 Pyrococcus furiosus 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin
-
 Thermoplasma acidophilum 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin
-
 Mycobacterium tuberculosis 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin
-
 Methanocaldococcus jannaschii 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin
-
 Archaeoglobus fulgidus 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin
-
 Thermotoga maritima 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin
-
 Lactobacillus leichmannii 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin
-
 Chloroflexus aurantiacus 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin
-
 Deinococcus radiodurans 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin
-
 Deinococcus radiodurans R1 / ATCC 13939 / DSM 20539 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin
-
 Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin + H2O
-
 Escherichia coli 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin + H2O
-
 Pyrococcus furiosus 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin + H2O
-
 Thermoplasma acidophilum 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin + H2O
-
 Mycobacterium tuberculosis 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin + H2O
-
 Methanocaldococcus jannaschii 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin + H2O
-
 Archaeoglobus fulgidus 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin + H2O
-
 Thermotoga maritima 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin + H2O
-
 Lactobacillus leichmannii 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin + H2O
-
 Chloroflexus aurantiacus 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin + H2O
-
 Deinococcus radiodurans 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin + H2O
-
 Deinococcus radiodurans R1 / ATCC 13939 / DSM 20539 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r
1.17.4.2 ribonucleoside triphosphate + reduced thioredoxin + H2O
-
 Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 2'-deoxyribonucleoside triphosphate + oxidized thioredoxin + H2O
-
 r

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.17.4.2 80 90
-
 Thermotoga maritima

Cofactor

EC Number Cofactor Comment Organism Structure
1.17.4.2 coenzyme B12
-
 Thermoplasma acidophilum
1.17.4.2 coenzyme B12
-
 Mycobacterium tuberculosis
1.17.4.2 coenzyme B12 adenosylcobalamin Pyrococcus furiosus
1.17.4.2 coenzyme B12 adenosylcobalamin Lactobacillus leichmannii