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Literature summary extracted from
- Phenylalanine 4-monooxygenase from Chromobacterium violaceum (1987), Methods Enzymol., 142, 44-49.
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.16.1 | 0.054 | - |
2-amino-4-hydroxy-6,7-dimethyltetrahydropteridine | - |
Chromobacterium violaceum |  |
| 1.14.16.1 | 0.14 | - |
L-phenylalanine | - |
Chromobacterium violaceum | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.14.16.1 | 31000 | 32400 | - |
Chromobacterium violaceum |
| 1.14.16.1 | 33000 | - |
1 * 33000, SDS-PAGE | Chromobacterium violaceum |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.16.1 | Chromobacterium violaceum | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.16.1 | protamine, DEAE-Sephadex, acid, DEAE-cellulose, Ultrogel, hydroxylapatite, Blue dextran-phenyl-butylamine-Sepharose | Chromobacterium violaceum |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.14.16.1 | 13.2 | - |
- |
Chromobacterium violaceum |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.14.16.1 | -20°C, 50 mM acetate, pH 6.0, 3 months, 20% loss of activity | Chromobacterium violaceum |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.16.1 | 3-phenylserine + tetrahydrobiopterin + O2 | - |
Chromobacterium violaceum | ? | - |
? | |
| 1.14.16.1 | L-phenylalanine + 2-amino-4-hydroxy-6,7-dimethyltetrahydropteridine | - |
Chromobacterium violaceum | ? | - |
r | |
| 1.14.16.1 | L-phenylalanine + tetrahydrobiopterin + O2 | additional electron donors: 2-amino-4-hydroxy-6,7-dimethyltetrahydropteridine, 2-amino-4-hydroxy-6-methyltetrahydropteridine, tetrahydrofolate, and 6-methyl-5-deazatetrahydropterin | Chromobacterium violaceum | L-tyrosine + dihydrobiopterin + H2O | - |
? | |
| 1.14.16.1 | L-tryptophan + tetrahydrobiopterin + O2 | 0.4% of activity with L-phenylalanine | Chromobacterium violaceum | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.14.16.1 | monomer | 1 * 33000, SDS-PAGE | Chromobacterium violaceum |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.16.1 | additional information | artificial cofactors: 2-amino-4-hydroxy-6,7-dimethyltetrahydropteridine, 6-methyl-5-deazatetrahydropterin and 2-amino-4-hydroxy-6-methyltetrahydropteridine can replace tetrahydrobiopterin | Chromobacterium violaceum | |
| 1.14.16.1 | tetrahydrobiopterin | - |
Chromobacterium violaceum | |
| 1.14.16.1 | tetrahydrofolate | - |
Chromobacterium violaceum | |
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