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Literature summary extracted from

  • Letendre, C.H.; Dickens, G.; Guroff, G.
    Phenylalanine hydroxylase from Pseudomonas sp. (ATCC 11299a). Purification, molecular weight, and influence of tyrosine metabolites on activation and hydroxylation (1975), J. Biol. Chem., 250, 6672-6678.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.16.1 4-hydroxyphenylacetic acid 0.4 mM, approx. 30% activation Pseudomonas sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.16.1 4-hydroxyphenylpyruvic acid above 0.4 mM iron, activation below Pseudomonas sp.
1.14.16.1 D,L-DOPA 0.1 mM, approx. 60% inhibition Pseudomonas sp.
1.14.16.1 D,L-m-tyrosine 0.4 mM, approx. 80% inhibition Pseudomonas sp.
1.14.16.1 dopamine 0.5 mM, approx. 40% inhibition Pseudomonas sp.
1.14.16.1 L-3,4-dihydroxyphenylalanine
-
 Pseudomonas sp.
1.14.16.1 L-Dopa 0.3 mM, approx. 40% inhibition Pseudomonas sp.
1.14.16.1 L-tryptophan
-
 Pseudomonas sp.
1.14.16.1 phenylalanine
-
 Pseudomonas sp.
1.14.16.1 tyrosine
-
 Pseudomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.16.1 25000 27000 sucrose density gradient centrifugation, gel filtration Pseudomonas sp.
1.14.16.1 28000
-
 1 * 28000, SDS-PAGE Pseudomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
1.14.16.1 Pseudomonas sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.16.1
-
 Pseudomonas sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.16.1 3.9
-
-
 Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.16.1 L-phenylalanine + tetrahydrobiopterin + O2
-
 Pseudomonas sp. L-tyrosine + dihydrobiopterin + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.16.1 monomer 1 * 28000, SDS-PAGE Pseudomonas sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.16.1 tetrahydrobiopterin
-
 Pseudomonas sp.

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.14.16.1 0.11
-
 phenylalanine competitive vs. iron Pseudomonas sp.
1.14.16.1 0.3
-
 L-tryptophan competitive vs. iron Pseudomonas sp.
1.14.16.1 0.3
-
 tyrosine competitive vs. iron Pseudomonas sp.