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Literature summary extracted from
- Bacterial metabolism of resorcinylic compounds: purification and properties of orcinol hydroxylase and resorcinol hydroxylase from Pseudomonas putida ORC (1976), Eur. J. Biochem., 61, 259-269.
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.14.13.6 | 68000 | - |
gel filtration | Pseudomonas putida |
| 1.14.13.6 | 70000 | - |
1 * 70000, SDS-PAGE | Pseudomonas putida |
| 1.14.14.27 | 68000 | - |
gel filtration | Pseudomonas putida |
| 1.14.14.27 | 70000 | - |
1 * 70000, SDS-PAGE | Pseudomonas putida |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.14.27 | resorcinol + FADH2 + O2 | Pseudomonas putida | the enzyme participates in the degradation of gamma-resorcylate and resorcinol | hydroxyquinol + FAD + H2O | - |
? |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.13.6 | Pseudomonas putida | - |
ORC | - |
| 1.14.13.6 | Pseudomonas putida ORC | - |
ORC | - |
| 1.14.14.27 | Pseudomonas putida | - |
- |
- |
| 1.14.14.27 | Pseudomonas putida ORC | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.13.6 | ORC, using protamine sulfate treatment, column chromatography on DEAE-cellulose, Sephadex G-100, Sephadex G-75 and a second DEAE-cellulose column chromatography | Pseudomonas putida |
| 1.14.14.27 | resorcinol hydroxylase accounts for about 3% of the total protein in Pseudomonas putida ORC after growth on resorcinol | Pseudomonas putida |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.14.13.6 | 11.7 | - |
- |
Pseudomonas putida |
| 1.14.14.27 | 11.6 | - |
pH 6.8, 30°C | Pseudomonas putida |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.13.6 | 3-chlorophenol + NADH + O2 | 33% of activity with orcinol | Pseudomonas putida | ? | - |
? | |
| 1.14.13.6 | 3-cresol + NADH + O2 | 11-14% of the activity with orcinol | Pseudomonas putida | 3-methylcatechol + NAD+ + H2O | - |
? | |
| 1.14.13.6 | 3-cresol + NADH + O2 | 11-14% of the activity with orcinol | Pseudomonas putida ORC | 3-methylcatechol + NAD+ + H2O | - |
? | |
| 1.14.13.6 | 3-ethylphenol + NADH + O2 | 30% of activity with orcinol | Pseudomonas putida | ? | - |
? | |
| 1.14.13.6 | 3-trifluoromethylphenol + NADH + O2 | 94% of activity with orcinol | Pseudomonas putida | ? | - |
? | |
| 1.14.13.6 | 3-trifluoromethylphenol + NADH + O2 | 94% of activity with orcinol | Pseudomonas putida ORC | ? | - |
? | |
| 1.14.13.6 | additional information | - |
Pseudomonas putida | ? | - |
? | |
| 1.14.13.6 | additional information | - |
Pseudomonas putida ORC | ? | - |
? | |
| 1.14.13.6 | orcinol + NADH + O2 | - |
Pseudomonas putida | 2,3,5-trihydroxytoluene + NAD+ + H2O | - |
? | |
| 1.14.13.6 | orcinol + NADH + O2 | - |
Pseudomonas putida ORC | 2,3,5-trihydroxytoluene + NAD+ + H2O | - |
? | |
| 1.14.13.6 | resorcinol + NADH + O2 | 23% of activity with orcinol | Pseudomonas putida | hydroxyquinol + NAD+ + H2O | - |
? | |
| 1.14.13.6 | resorcinol + NADH + O2 | 23% of activity with orcinol | Pseudomonas putida ORC | hydroxyquinol + NAD+ + H2O | - |
? | |
| 1.14.14.27 | orcinol + FADH2 + O2 | 90% of the hydroxylation activity as compared to resorcinol | Pseudomonas putida | ? + FAD + H2O | - |
? | |
| 1.14.14.27 | resorcinol + FADH2 + O2 | - |
Pseudomonas putida | hydroxyquinol + FAD + H2O | - |
? | |
| 1.14.14.27 | resorcinol + FADH2 + O2 | the enzyme participates in the degradation of gamma-resorcylate and resorcinol | Pseudomonas putida | hydroxyquinol + FAD + H2O | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.14.13.6 | monomer | 1 * 70000, SDS-PAGE | Pseudomonas putida |
| 1.14.14.27 | monomer | 1 * 70000, SDS-PAGE | Pseudomonas putida |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.14.27 | graA | - |
Pseudomonas putida |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.13.6 | 30 | - |
assay at | Pseudomonas putida |
| 1.14.14.27 | 30 | - |
assay at | Pseudomonas putida |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.13.6 | 6.8 | - |
assay at | Pseudomonas putida |
| 1.14.14.27 | 6.8 | - |
assay at | Pseudomonas putida |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.13.6 | FAD | flavoprotein | Pseudomonas putida | |
| 1.14.13.6 | FAD | 1 mol of FAD per mol of protein | Pseudomonas putida | |
| 1.14.13.6 | NADH | - |
Pseudomonas putida | |
| 1.14.13.6 | NADPH | - |
Pseudomonas putida | |
| 1.14.14.27 | FADH2 | contains approximately 1 mol of FAD for each polypeptide chain. The enzyme uses FADH2 as a substrate rather than a cofactor. FADH2 is provided by flavin reductase (NADH) | Pseudomonas putida | |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 1.14.14.27 | metabolism | the enzyme participates in the degradation of gamma-resorcylate and resorcinol | Pseudomonas putida |
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