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Literature summary extracted from

  • Meyer, A.; Wursten, M.; Schmid, A.; Kohler, H.P.; Witholt, B.
    Hydroxylation of indole by laboratory-evolved 2-hydroxybiphenyl 3-monooxygenase (2002), J. Biol. Chem., 277, 34161-34167.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.14.13.44 additional information direct enzyme evolution of EC 1.14.13.44 results in an enzyme variant HbpAind, that hydroxylates indole and indole derivatives such as hydroxyindoles and 5-bromoindole. The wild-type protein does not catalyze theses reactions. HbpAind contains amino acid substitutions D222V and V368A. The activity for indole hydroxylation is increased 18fold in this variant Pseudomonas nitroreducens

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.44 Pseudomonas nitroreducens
-
 HBP1
-
1.14.13.44 Pseudomonas nitroreducens HBP1
-
 HBP1
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.44 2-hydroxybiphenyl + NADH + O2 the activity of the mutant enzyme HbpAind is six times lower than that of the wild-type enzyme Pseudomonas nitroreducens 2,3-dihydroxybiphenyl + NAD+ + H2O
-
 ?
1.14.13.44 2-hydroxybiphenyl + NADH + O2 the activity of the mutant enzyme HbpAind is six times lower than that of the wild-type enzyme Pseudomonas nitroreducens HBP1 2,3-dihydroxybiphenyl + NAD+ + H2O
-
 ?
1.14.13.44 indole + NADH + O2
-
 Pseudomonas nitroreducens ?
-
 ?
1.14.13.44 indole + NADH + O2
-
 Pseudomonas nitroreducens HBP1 ?
-
 ?
1.14.13.44 additional information Asp222 is involved in substrate activation in HbpA Pseudomonas nitroreducens ?
-
 ?
1.14.13.44 additional information Asp222 is involved in substrate activation in HbpA Pseudomonas nitroreducens HBP1 ?
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.13.44 0.005
-
 indole wild-type enzyme Pseudomonas nitroreducens
1.14.13.44 0.09
-
 indole mutant enzyme HbpAind Pseudomonas nitroreducens
1.14.13.44 2.3
-
 2-Hydroxybiphenyl mutant enzyme HbpAind Pseudomonas nitroreducens
1.14.13.44 15.6
-
 2-Hydroxybiphenyl wild-type enzyme Pseudomonas nitroreducens

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.44 NADH
-
 Pseudomonas nitroreducens