EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.14.13.44 | 2,3-Dihydroxybiphenyl | inhibits reaction with 2-hydroxybiphenyl | Pseudomonas nitroreducens | |
1.14.13.44 | AgNO3 | 0.01 mM, complete inhibition | Pseudomonas nitroreducens | |
1.14.13.44 | CuSO4 | 0.01 mM, complete inhibition | Pseudomonas nitroreducens | |
1.14.13.44 | FeSO4 | 0.08 mM, 30% inhibition | Pseudomonas nitroreducens | |
1.14.13.44 | HgCl2 | 0.01 mM, complete inhibition | Pseudomonas nitroreducens | |
1.14.13.44 | NaCl | 10 mM, 36% inhibition. 100 mM, 89% inhibition | Pseudomonas nitroreducens | |
1.14.13.44 | p-hydroxymercuribenzoate | partial protection in presence of 2-hydroxybiphenyl, reversed by excess of dithiothreitol | Pseudomonas nitroreducens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.13.44 | 0.0028 | - |
2-Hydroxybiphenyl | reaction with NADH and O2 | Pseudomonas nitroreducens | |
1.14.13.44 | 0.0031 | - |
2-Hydroxybiphenyl | reaction with NADPH and O2 | Pseudomonas nitroreducens | |
1.14.13.44 | 0.0034 | - |
2,2'-Dihydroxybiphenyl | reaction with NADPH and O2 | Pseudomonas nitroreducens | |
1.14.13.44 | 0.004 | - |
2,2'-Dihydroxybiphenyl | reaction with NADH and O2 | Pseudomonas nitroreducens | |
1.14.13.44 | 0.0057 | - |
2-sec-Butylphenol | reaction with NADH or NADPH and O2 | Pseudomonas nitroreducens | |
1.14.13.44 | 0.0216 | - |
NADH | reaction with 2,2'-dihydroxybiphenyl and O2 | Pseudomonas nitroreducens | |
1.14.13.44 | 0.0268 | - |
NADH | reaction with 2-hydroxybiphenyl and O2 | Pseudomonas nitroreducens | |
1.14.13.44 | 0.0292 | - |
O2 | reaction with 2-hydroxybiphenyl and NADH | Pseudomonas nitroreducens | |
1.14.13.44 | 0.0943 | - |
NADPH | reaction with 2,2'-dihydroxybiphenyl and O2 | Pseudomonas nitroreducens | |
1.14.13.44 | 0.137 | - |
NADPH | reaction with 2-hydroxybiphenyl and O2 | Pseudomonas nitroreducens |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.14.13.44 | 60000 | - |
4 * 60000, SDS-PAGE | Pseudomonas nitroreducens |
1.14.13.44 | 256000 | - |
gel filtration | Pseudomonas nitroreducens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.13.44 | 2-hydroxybiphenyl + NADH + O2 | Pseudomonas nitroreducens | first enzyme of 2-hydroxybiphenyl degradation | 2,3-dihydroxybiphenyl + NAD+ + H2O | - |
? | |
1.14.13.44 | 2-hydroxybiphenyl + NADH + O2 | Pseudomonas nitroreducens HBP1 | first enzyme of 2-hydroxybiphenyl degradation | 2,3-dihydroxybiphenyl + NAD+ + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.13.44 | Pseudomonas nitroreducens | - |
HBP1 | - |
1.14.13.44 | Pseudomonas nitroreducens HBP1 | - |
HBP1 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.14.13.44 | native and recombinant enzyme | Pseudomonas nitroreducens |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.14.13.44 | additional information | - |
- |
Pseudomonas nitroreducens |
EC Number | Storage Stability | Organism |
---|---|---|
1.14.13.44 | -20°C, pure enzyme at concentration of 3.8 mg/ml in 50 mM phosphate buffer, pH 7.5, stable for at least 6 months | Pseudomonas nitroreducens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.13.44 | 2,2'-dihydroxybiphenyl + NADH + O2 | - |
Pseudomonas nitroreducens | 2,2',3-trihydroxybiphenyl + NAD+ + H2O | - |
? | |
1.14.13.44 | 2,2'-dihydroxybiphenyl + NADPH + O2 | - |
Pseudomonas nitroreducens | 2,2',3-trihydroxybiphenyl + NADP+ + H2O | - |
? | |
1.14.13.44 | 2,5-dihydroxybiphenyl + NADH + O2 | - |
Pseudomonas nitroreducens | 2,3,5-trihydroxybiphenyl + NAD+ + H2O | - |
? | |
1.14.13.44 | 2-ethylphenol + NADH + O2 | - |
Pseudomonas nitroreducens | 1,2-dihydroxy-3-ethylbenzene + NAD+ + H2O | - |
? | |
1.14.13.44 | 2-hydroxybiphenyl + NADH + O2 | - |
Pseudomonas nitroreducens | 2,3-dihydroxybiphenyl + NAD+ + H2O | - |
? | |
1.14.13.44 | 2-hydroxybiphenyl + NADH + O2 | first enzyme of 2-hydroxybiphenyl degradation | Pseudomonas nitroreducens | 2,3-dihydroxybiphenyl + NAD+ + H2O | - |
? | |
1.14.13.44 | 2-hydroxybiphenyl + NADH + O2 | - |
Pseudomonas nitroreducens HBP1 | 2,3-dihydroxybiphenyl + NAD+ + H2O | - |
? | |
1.14.13.44 | 2-hydroxybiphenyl + NADH + O2 | first enzyme of 2-hydroxybiphenyl degradation | Pseudomonas nitroreducens HBP1 | 2,3-dihydroxybiphenyl + NAD+ + H2O | - |
? | |
1.14.13.44 | 2-hydroxybiphenyl + NADPH + O2 | - |
Pseudomonas nitroreducens | 2,3-dihydroxybiphenyl + NADP+ + H2O | - |
? | |
1.14.13.44 | 2-hydroxybiphenyl + NADPH + O2 | - |
Pseudomonas nitroreducens HBP1 | 2,3-dihydroxybiphenyl + NADP+ + H2O | - |
? | |
1.14.13.44 | 2-methylphenol + NADH + O2 | - |
Pseudomonas nitroreducens | 1,2-dihydroxy-3-methylbenzene + NAD+ + H2O | - |
? | |
1.14.13.44 | 2-propylphenol + NADH + O2 | - |
Pseudomonas nitroreducens | 1,2-dihydroxy-3-propylbenzene + NAD+ + H2O | - |
? | |
1.14.13.44 | 2-propylphenol + NADH + O2 | - |
Pseudomonas nitroreducens HBP1 | 1,2-dihydroxy-3-propylbenzene + NAD+ + H2O | - |
? | |
1.14.13.44 | 2-sec-butylphenol + NADH + O2 | - |
Pseudomonas nitroreducens | 2-sec-butylcatechol + NAD+ + H2O | - |
? | |
1.14.13.44 | 2-sec-butylphenol + NADPH + O2 | - |
Pseudomonas nitroreducens | 2-sec-butylcatechol + NADP+ + H2O | - |
? | |
1.14.13.44 | 2-tert-butylphenol + NADH + O2 | - |
Pseudomonas nitroreducens | 1,2-dihydroxy-3-tert-butylbenzene + NAD+ + H2O | - |
? | |
1.14.13.44 | additional information | the substrates partially uncouple oxygen activation from hydroxylation with resultant reduction of both atoms of oxygen to form hydrogen peroxide | Pseudomonas nitroreducens | ? | - |
? | |
1.14.13.44 | additional information | the substrates partially uncouple oxygen activation from hydroxylation with resultant reduction of both atoms of oxygen to form hydrogen peroxide | Pseudomonas nitroreducens HBP1 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.14.13.44 | tetramer | 4 * 60000, SDS-PAGE | Pseudomonas nitroreducens |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.13.44 | 9 | - |
2,2'-Dihydroxybiphenyl | reaction with NADH and O2 | Pseudomonas nitroreducens | |
1.14.13.44 | 9.4 | - |
2,2'-Dihydroxybiphenyl | reaction with NADPH and O2 | Pseudomonas nitroreducens | |
1.14.13.44 | 9.8 | - |
NADH | reaction with 2,2'-dihydroxybiphenyl and O2 | Pseudomonas nitroreducens | |
1.14.13.44 | 10.2 | - |
2-sec-Butylphenol | reaction with NADPH and O2 | Pseudomonas nitroreducens | |
1.14.13.44 | 11.2 | - |
NADPH | reaction with 2,2'-dihydroxybiphenyl and O2 | Pseudomonas nitroreducens | |
1.14.13.44 | 12.8 | - |
2-Hydroxybiphenyl | reaction with NADPH and O2 | Pseudomonas nitroreducens | |
1.14.13.44 | 14.9 | - |
2-Hydroxybiphenyl | reaction with NADH and O2 | Pseudomonas nitroreducens | |
1.14.13.44 | 15.8 | - |
2-sec-Butylphenol | reaction with NADH and O2 | Pseudomonas nitroreducens | |
1.14.13.44 | 16.2 | - |
NADH | reaction with 2-hydroxybiphenyl and O2 | Pseudomonas nitroreducens | |
1.14.13.44 | 16.2 | - |
O2 | reaction with 2-hydroxybiphenyl and NADH | Pseudomonas nitroreducens | |
1.14.13.44 | 18.8 | - |
NADPH | reaction with 2-hydroxybiphenyl and O2 | Pseudomonas nitroreducens |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.14.13.44 | 7.5 | - |
- |
Pseudomonas nitroreducens |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.14.13.44 | 7.2 | 7.8 | more than 80% of maximal activity at pH 7.2 and pH 7.8, beyond pH 7.8 activity declines abruptly with increasing pH | Pseudomonas nitroreducens |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.13.44 | FAD | one molecule of noncovalently bound FAD per subunit | Pseudomonas nitroreducens | |
1.14.13.44 | NADH | - |
Pseudomonas nitroreducens | |
1.14.13.44 | NADPH | can replace NADH as electron donor, Km-value for NADPH is much higher than for NADH | Pseudomonas nitroreducens |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.13.44 | 0.9 | - |
2,3-Dihydroxybiphenyl | - |
Pseudomonas nitroreducens |