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Literature summary extracted from

  • Sreeleela, N.S.; SubraRao, P.V.; Premkumar, R.; Vaidyanathan, C.S.
    A new anthranilic acid hydroxylase from Aspergillus niger. Purification and properties (1969), J. Biol. Chem., 244, 2293-2298.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.14.13.35 glutathione stabilizes Aspergillus niger
1.14.13.35 the enzyme is inactivated by treatment with ammonium sulfate or by adsorption on DEAE-cellulose or CM-cellulose, filtration through Sephadex G-25 or dialysis against 0.025 M sodium phosphate buffer, pH 7, containing 1 mM GSH irreversibly inactivates the enzyme Aspergillus niger

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.13.35 1,10-phenanthroline 46% inhibition at 0.5 mM, 60% inhibition at 1 mM Aspergillus niger
1.14.13.35 2,2'-dipyridyl 40% inhibition at 1 mM Aspergillus niger
1.14.13.35 3-hydroxyanthranilic acid
-
 Aspergillus niger
1.14.13.35 3-Hydroxybenzoic acid
-
 Aspergillus niger
1.14.13.35 8-hydroxyquinoline 37% inhibition at 1 mM Aspergillus niger
1.14.13.35 Cd2+
-
 Aspergillus niger
1.14.13.35 Cu2+
-
 Aspergillus niger
1.14.13.35 diethyldithiocarbamate
-
 Aspergillus niger
1.14.13.35 heavy metal ions
-
 Aspergillus niger
1.14.13.35 Hg2+
-
 Aspergillus niger
1.14.13.35 additional information not: atebrin, aminopterin Aspergillus niger
1.14.13.35 N-ethylmaleimide 95% inhibition at 0.5 mM Aspergillus niger
1.14.13.35 NaN3 42% inhibition at 0.5 mM Aspergillus niger
1.14.13.35 p-hydroxymercuribenzoate 96% inhibition at 0.5 mM Aspergillus niger
1.14.13.35 Salicylaldoxime 42% inhibition at 0.5 mM, 58% inhibition at 1 mM Aspergillus niger

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.13.35 0.15
-
 anthranilic acid
-
 Aspergillus niger
1.14.13.35 0.16
-
 NADPH
-
 Aspergillus niger

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.13.35 Iron Fe2+ probably required for activity Aspergillus niger

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
1.14.13.35 Acetone inactivates the enzyme Aspergillus niger

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.35 Aspergillus niger
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.35 partial, using protamine sulfate treatment, DEAE-cellulose treatment, alumina C-gamma treatment and hydroxylapatite treatment Aspergillus niger

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.13.35 mycelium
-
 Aspergillus niger
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.13.35 0.0507
-
-
 Aspergillus niger
1.14.13.35 0.0507
-
 partially purified enzyme Aspergillus niger

Storage Stability

EC Number Storage Stability Organism
1.14.13.35 -20°C, 50% inactivation of the purified enzyme after 2 days Aspergillus niger

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.35 anthranilate + NADPH + O2
-
 Aspergillus niger 2,3-dihydroxybenzoate + NADP+ + NH3
-
 ?
1.14.13.35 additional information no activity with: 3-hydroxyanthranilic acid, benzoic acid, salicylic acid, m-hydroxybenzoic acid, p-hydroxybenzoic acid, m-aminobenzoic acid, p-aminobenzoic acid, methylanthranilate or ethylanthranilate Aspergillus niger ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.13.35 8.2
-
-
 Aspergillus niger

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.14.13.35 7.5 9.5 pH 7.5: about 80% of activity maximum, pH 9.5: about 55% of activity maximum Aspergillus niger

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.35 additional information 2% of the activity when NADPH is replaced by NADH, no effect: FAD, FMN Aspergillus niger
1.14.13.35 NADPH absolute requirement for NADPH Aspergillus niger