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Literature summary extracted from
- Pseudomonas cepacia 3-hydroxybenzoate 6-hydroxylase: induction, purification, and characterization (1987), Biochemistry, 26, 1099-1104.
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.13.24 | 0.04 | - |
NADH | cosubstrate 3-hydroxybenzoate | Burkholderia cepacia |  |
| 1.14.13.24 | 0.064 | - |
NADPH | cosubstrate 3-hydroxybenzoate | Burkholderia cepacia | |
| 1.14.13.24 | 0.162 | - |
3-hydroxybenzoate | cosubstrate NADH | Burkholderia cepacia | |
| 1.14.13.24 | 0.168 | - |
3-hydroxybenzoate | cosubstrate NADPH | Burkholderia cepacia | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.14.13.24 | 40000 | 45000 | gel filtration, SDS-PAGE | Burkholderia cepacia |
| 1.14.13.24 | 44000 | - |
1 * 44000, SDS-PAGE | Burkholderia cepacia |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.13.24 | Burkholderia cepacia | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.13.24 | homogeneity | Burkholderia cepacia |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.14.13.24 | 23.7 | - |
- |
Burkholderia cepacia |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.13.24 | 3-hydroxybenzoate + NADH + O2 | - |
Burkholderia cepacia | 2,5-dihydroxybenzoate + NAD+ + H2O | - |
? | |
| 1.14.13.24 | 3-hydroxybenzoate + NADPH + H+ + O2 | - |
Burkholderia cepacia | 2,5-dihydroxybenzoate + NADP+ + H2O | - |
r | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.14.13.24 | monomer | 1 * 44000, SDS-PAGE | Burkholderia cepacia |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.13.24 | 8 | - |
- |
Burkholderia cepacia |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.13.24 | FAD | flavoprotein | Burkholderia cepacia | |
| 1.14.13.24 | FAD | 1 mol of FAD per mol of enzyme | Burkholderia cepacia | |
| 1.14.13.24 | NADH | - |
Burkholderia cepacia | |
| 1.14.13.24 | NADPH | NADH and NADPH utilized with similar efficiencies | Burkholderia cepacia | |
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