EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.14.13.22 | 7-Chloro-8-demethyl-FAD | reactivates apoenzyme | Acinetobacter sp. |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.14.13.22 | 5,5'-dithiobis(2-nitrobenzoate) | - |
Acinetobacter sp. | |
1.14.13.22 | 5-deaza-FAD | competitive | Acinetobacter sp. | |
1.14.13.22 | p-hydroxymercuribenzoate | - |
Acinetobacter sp. |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.13.22 | 0.004 | - |
cyclohexanone | - |
Acinetobacter sp. | |
1.14.13.22 | 0.02 | - |
NADPH | - |
Acinetobacter sp. | |
1.14.13.22 | 3.6 | - |
Cyclopentanone | - |
Acinetobacter sp. |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.13.22 | cyclohexanone + NADPH + O2 | Acinetobacter sp. | enzyme plays an important role in degradation of keto-compounds by microorganisms | 1-oxa-2-oxocycloheptane + NADP+ + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.13.22 | Acinetobacter sp. | - |
NCIB 9871 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.14.13.22 | - |
Acinetobacter sp. |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.14.13.22 | cyclohexanone + NADPH + H+ + O2 = hexano-6-lactone + NADP+ + H2O | ter-ter mechanism | Acinetobacter sp. | |
1.14.13.22 | cyclohexanone + NADPH + H+ + O2 = hexano-6-lactone + NADP+ + H2O | a stable 4a-hydroperoxy-flavin carries out a nucleophilic attack on the ketone | Acinetobacter sp. |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.14.13.22 | 2.1 | - |
- |
Acinetobacter sp. |
EC Number | Storage Stability | Organism |
---|---|---|
1.14.13.22 | -20°C, more than 1 year | Acinetobacter sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.13.22 | (+)-camphor + NADPH + O2 | - |
Acinetobacter sp. | ? | - |
? | |
1.14.13.22 | 2-methylcyclohexanone + NADPH + O2 | - |
Acinetobacter sp. | 1-oxa-2-oxo-3-methylcycloheptane + NADP+ + H2O | - |
? | |
1.14.13.22 | 4-methylcyclohexanone + NADPH + O2 | - |
Acinetobacter sp. | 1-oxa-2-oxo-5-methylcycloheptane + NADP+ + H2O | - |
? | |
1.14.13.22 | cyclobutanone + NADPH + O2 | - |
Acinetobacter sp. | 1-oxa-2-oxo-cyclopentane + NADP+ + H2O | - |
? | |
1.14.13.22 | cycloheptanone + NADPH + O2 | - |
Acinetobacter sp. | 1-oxa-2-oxo-cyclooctane + NADP+ + H2O | - |
? | |
1.14.13.22 | cyclohexanone + NADPH + O2 | - |
Acinetobacter sp. | 6-hexanolide + NADP+ + H2O | product: epsilon-caprolactone i.e. 1-oxa-2-oxocycloheptane | ? | |
1.14.13.22 | cyclohexanone + NADPH + O2 | enzyme plays an important role in degradation of keto-compounds by microorganisms | Acinetobacter sp. | 1-oxa-2-oxocycloheptane + NADP+ + H2O | - |
? | |
1.14.13.22 | cyclooctanone + NADPH + O2 | - |
Acinetobacter sp. | 1-oxa-2-oxo-cyclononane + NADP+ + H2O | - |
? | |
1.14.13.22 | cyclopentanone + NADPH + O2 | - |
Acinetobacter sp. | 1-oxa-2-oxo-cyclohexane + NADP+ + H2O | - |
? | |
1.14.13.22 | dihydrocarvone + NADPH + O2 | - |
Acinetobacter sp. | ? | - |
? | |
1.14.13.22 | additional information | absolutely specific for NADPH as electron donor | Acinetobacter sp. | ? | - |
? | |
1.14.13.22 | norcamphor + NADPH + O2 | - |
Acinetobacter sp. | ? | - |
? |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.14.13.22 | 9 | - |
- |
Acinetobacter sp. |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.13.22 | 6-Methyl-FAD | reactivates apoenzyme | Acinetobacter sp. | |
1.14.13.22 | 9-Aza-FAD | reactivates apoenzyme | Acinetobacter sp. | |
1.14.13.22 | FAD | - |
Acinetobacter sp. | |
1.14.13.22 | NADPH | electron donor | Acinetobacter sp. | |
1.14.13.22 | NADPH | absolutely specific for NADPH as electron donor | Acinetobacter sp. |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.13.22 | 0.062 | - |
5-deaza-FAD | - |
Acinetobacter sp. |