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Literature summary extracted from

  • Griffin, M.; Trudgill, P.W.
    Purification and properties of cyclopentanone oxygenase of Pseudomonas NCIB 9872 (1976), Eur. J. Biochem., 63, 199-209.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.13.16 5,5'-dithiobis(2-nitrobenzoate)
-
 Pseudomonas sp.
1.14.13.16 arsenite
-
 Pseudomonas sp.
1.14.13.16 Bathocuproine
-
 Pseudomonas sp.
1.14.13.16 bathophenanthroline
-
 Pseudomonas sp.
1.14.13.16 diethyldithiocarbamate
-
 Pseudomonas sp.
1.14.13.16 iodoacetamide
-
 Pseudomonas sp.
1.14.13.16 p-hydroxymercuribenzoate
-
 Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.13.16 0.001
-
 O2
-
 Pseudomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.13.16 200000
-
 gel filtration, sedimentation velocity Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.13.16 cyclopentanone + NADPH + O2 Pseudomonas sp.
-
 5-valerolactone + NADP+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.16 Pseudomonas sp.
-
 NCIB 9872
-

Oxidation Stability

EC Number Oxidation Stability Organism
1.14.13.16 reduction of enzyme by visible light in presence of EDTA Pseudomonas sp.

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.16
-
 Pseudomonas sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.13.16 4.3 5
-
 Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.16 2-methylcyclohexanone + NADPH + O2
-
 Pseudomonas sp. 1-oxa-2-oxo-3-methylcycloheptane + NADP+ + H2O
-
 ?
1.14.13.16 butan-2-one + NADPH + O2
-
 Pseudomonas sp. ?
-
 ?
1.14.13.16 cyclobutanone + NADPH + O2
-
 Pseudomonas sp. butyrolactone + NADP+ + H2O
-
 ?
1.14.13.16 cycloheptanone + NADPH + O2 poor substrate Pseudomonas sp. 1-oxa-2-oxocyclooctane + NADP+ + H2O
-
 ?
1.14.13.16 cyclohexanone + NADPH + O2
-
 Pseudomonas sp. 1-oxa-2-oxocycloheptane + NADP+ + H2O
-
 ?
1.14.13.16 cyclooctanone + NADPH + O2 poor substrate Pseudomonas sp. 1-oxa-2-oxocyclononane + NADP+ + H2O
-
 ?
1.14.13.16 cyclopentanone + NADPH + O2
-
 Pseudomonas sp. 5-valerolactone + NADP+ + H2O
-
 ?
1.14.13.16 norbornanone + NADPH + O2
-
 Pseudomonas sp. ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.13.16 trimer or tetramer 3 or 4 * 54000-58000, SDS-PAGE Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.13.16 7.7
-
-
 Pseudomonas sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.16 FAD FAD : enzyme ratio is 2.2 to 3.5 Pseudomonas sp.
1.14.13.16 NADPH
-
 Pseudomonas sp.