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Literature summary extracted from
- Purification and characterization of 5beta-cholestane-3alpha,7alpha,12alpha-triol 27-hydroxylase from female rat liver mitochondria (1988), J. Biol. Chem., 263, 18138-18142.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.15.15 | 7,8-Benzoflavone | 68% inhibition at 0.04 mM | Rattus norvegicus |  |
| 1.14.15.15 | Aminoglutethimide | slight inhibition | Rattus norvegicus | |
| 1.14.15.15 | CO | - |
Rattus norvegicus | |
| 1.14.15.15 | p-chloromercuribenzoate | complete inhibition at 0.08 mM | Rattus norvegicus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.15.15 | 0.0063 | - |
5beta-cholestane-3alpha,7alpha,12alpha-triol | - |
Rattus norvegicus | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.14.15.15 | mitochondrion | - |
Rattus norvegicus | 5739 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.14.15.15 | 52500 | - |
SDS-PAGE | Rattus norvegicus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.15.15 | Rattus norvegicus | - |
female rat | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.15.15 | - |
Rattus norvegicus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.14.15.15 | liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.14.15.15 | 0.431 | - |
- |
Rattus norvegicus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.15.15 | 5beta-cholestane-3alpha,7alpha,12alpha-triol + reduced adrenodoxin + O2 | - |
Rattus norvegicus | 5beta-cholestane-3alpha,7alpha,12alpha,27-tetrol + oxidized adrenodoxin + H2O | - |
? | |
| 1.14.15.15 | 5beta-cholestane-3alpha,7alpha-diol + reduced adrenodoxin + O2 | - |
Rattus norvegicus | ? | - |
? | |
| 1.14.15.15 | cholesterol + reduced adrenodoxin + O2 | - |
Rattus norvegicus | ? | - |
? | |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.15.15 | 0.0117 | - |
cholesterol | - |
Rattus norvegicus | |
| 1.14.15.15 | 0.217 | - |
5beta-cholestane-3alpha,7alpha-diol | - |
Rattus norvegicus | |
| 1.14.15.15 | 0.592 | - |
5beta-cholestane-3alpha,7alpha,12alpha-triol | - |
Rattus norvegicus | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.15.15 | 7.8 | - |
- |
Rattus norvegicus |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.15.15 | cytochrome P450 | - |
Rattus norvegicus |
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