EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.13.10 | expressed in Escherichia coli XL-1 blue | Paenarthrobacter nicotinovorans |
EC Number | General Stability | Organism |
---|---|---|
1.14.13.10 | dialysis against 1.5 M guanidinium hydrochloride removes FAD, no renaturation possible | Paenarthrobacter nicotinovorans |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.14.13.10 | 2,3-dihydroxypyridine | 100% inhibition at 0.1 mM, irreversible inhibitor | Paenarthrobacter nicotinovorans | |
1.14.13.10 | 2,6-dihydroxynicotinamide | 55% inhibition at 0.1 mM, reversible inhibitor | Paenarthrobacter nicotinovorans | |
1.14.13.10 | 2,6-dimethoxypyridine | 100% inhibition at 0.1 mM, irreversible inhibitor | Paenarthrobacter nicotinovorans | |
1.14.13.10 | 2-Hydroxypyridine | 48% inhibition at 0.1 mM, reversible inhibitor | Paenarthrobacter nicotinovorans | |
1.14.13.10 | resorcine | 51% inhibition at 0.1 mM, reversible inhibitor | Paenarthrobacter nicotinovorans |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.13.10 | 0.0083 | - |
2,6-Dihydroxypyridine | pH 8, 20°C | Paenarthrobacter nicotinovorans |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.14.13.10 | 90000 | - |
gel filtration | Paenarthrobacter nicotinovorans |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.13.10 | 2,6-dihydroxypyridine + NADH + O2 | Paenarthrobacter nicotinovorans | - |
2,3,6-trihydroxypyridine + NAD+ + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.13.10 | Paenarthrobacter nicotinovorans | - |
formerly Arthrobacter oxidans | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.14.13.10 | - |
Paenarthrobacter nicotinovorans |
EC Number | Renatured (Comment) | Organism |
---|---|---|
1.14.13.10 | after removal of FAD through precipitation with 50% (NH4)2SO4 at pH 2.0, incubation with 0.1 mM FAD leeds to 90% activity recovery | Paenarthrobacter nicotinovorans |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.14.13.10 | 90 | - |
- |
Paenarthrobacter nicotinovorans |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.13.10 | 2,6-dihydroxypyridine + NADH + O2 | - |
Paenarthrobacter nicotinovorans | 2,3,6-trihydroxypyridine + NAD+ + H2O | - |
? | |
1.14.13.10 | 2,6-dihydroxypyridine + NADH + O2 | - |
Paenarthrobacter nicotinovorans | 2,3,6-trihydroxypyridine + NAD+ + H2O | product dimerizes spontaneously in the presence of oxygen to form a blue pigment | ? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.14.13.10 | homodimer | - |
Paenarthrobacter nicotinovorans |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.14.13.10 | 20 | - |
- |
Paenarthrobacter nicotinovorans |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.14.13.10 | 4 | - |
stable | Paenarthrobacter nicotinovorans |
1.14.13.10 | 52 | - |
inactivation | Paenarthrobacter nicotinovorans |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.14.13.10 | 8 | - |
- |
Paenarthrobacter nicotinovorans |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.13.10 | FAD | 2 mol FAD per dimer | Paenarthrobacter nicotinovorans | |
1.14.13.10 | NADH | no reaction with NADPH | Paenarthrobacter nicotinovorans |