EC Number | General Stability | Organism |
---|---|---|
1.14.12.9 | 75% loss of activity after 16 h dialysis | Pseudomonas sp. |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.14.12.9 | 1,10-phenanthroline | 1 mM, 34% inhibition | Pseudomonas sp. | |
1.14.12.9 | EDTA | 1 mM, 32% inhibition | Pseudomonas sp. | |
1.14.12.9 | iodoacetate | 0.01 mM, 77% inhibition | Pseudomonas sp. | |
1.14.12.9 | KCN | 1 mM, 52% inhibition | Pseudomonas sp. | |
1.14.12.9 | NEM | 1 mM, 38% inhibition | Pseudomonas sp. | |
1.14.12.9 | NH4+ | inhibition of activity of reductase component | Pseudomonas sp. | |
1.14.12.9 | PCMB | 0.005 mM, complete inhibition | Pseudomonas sp. |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.12.9 | 0.0023 | - |
ferrocytochrome c | - |
Pseudomonas sp. | |
1.14.12.9 | 0.0063 | - |
reduced 2,6-dichlorophenolindophenol | - |
Pseudomonas sp. | |
1.14.12.9 | 0.032 | - |
NADH | - |
Pseudomonas sp. |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.14.12.9 | Iron | reductase component contains an iron-sulfur cluster of the [2Fe-2S]-type, contains 2.1 mol of iron and 1.7 mol of acid-labile sulfide per mol of reductase | Pseudomonas sp. |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.14.12.9 | 35000 | - |
reductase component of 4-chlorophenylacetate 3,4-dioxygenase, gel filtration | Pseudomonas sp. |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.12.9 | Pseudomonas sp. | - |
- |
- |
1.14.12.9 | Pseudomonas sp. CBS 3 | - |
- |
- |
EC Number | Oxidation Stability | Organism |
---|---|---|
1.14.12.9 | reductase component loses activity in presence of molecular oxygen | Pseudomonas sp. |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.14.12.9 | reductase component | Pseudomonas sp. |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.14.12.9 | 30 | - |
reductase component of of 4-chlorophenylacetate 3,4-dioxygenase | Pseudomonas sp. |
EC Number | Storage Stability | Organism |
---|---|---|
1.14.12.9 | 4°C, 20 mM potassium phosphate buffer, pH 6.0-7.5, 10 days, 50% loss of activity | Pseudomonas sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.12.9 | 4-chlorophenylacetate + ferrocytochrome c + H+ + O2 | - |
Pseudomonas sp. | 3,4-dihydroxyphenylacetate + chloride + ferricytochrome c | - |
r | |
1.14.12.9 | 4-chlorophenylacetate + ferrocytochrome c + H+ + O2 | - |
Pseudomonas sp. CBS 3 | 3,4-dihydroxyphenylacetate + chloride + ferricytochrome c | - |
r | |
1.14.12.9 | 4-chlorophenylacetate + NADH + O2 + H2O | - |
Pseudomonas sp. | 3,4-dihydroxyphenylacetate + Cl- + NAD(P)+ | - |
? | |
1.14.12.9 | 4-chlorophenylacetate + NADH + O2 + H2O | - |
Pseudomonas sp. CBS 3 | 3,4-dihydroxyphenylacetate + Cl- + NAD(P)+ | - |
? | |
1.14.12.9 | 4-chlorophenylacetate + reduced 2,6-dichlorophenolindophenol + O2 | - |
Pseudomonas sp. | 3,4-dihydroxyphenylacetate + chloride + oxidized 2,6-dichlorophenolindophenol | - |
r | |
1.14.12.9 | 4-chlorophenylacetate + reduced 2,6-dichlorophenolindophenol + O2 | - |
Pseudomonas sp. CBS 3 | 3,4-dihydroxyphenylacetate + chloride + oxidized 2,6-dichlorophenolindophenol | - |
r | |
1.14.12.9 | additional information | the reductase component reduces several redox compounds: 2,6-dichlorophenylindophenol, potassium hexacyanoferrate III, cytochrome c, methylene blue and nitro blue tetrazolium | Pseudomonas sp. | ? | - |
? | |
1.14.12.9 | additional information | the reductase component reduces several redox compounds: 2,6-dichlorophenylindophenol, potassium hexacyanoferrate III, cytochrome c, methylene blue and nitro blue tetrazolium | Pseudomonas sp. CBS 3 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.14.12.9 | monomer | 1 * 35000, reductase component of of 4-chlorophenylacetate 3,4-dioxygenase, SDS-PAGE | Pseudomonas sp. |
1.14.12.9 | More | the enzyme consists of two components, a monomeric reductase and a trimeric dioxygenase | Pseudomonas sp. |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.14.12.9 | 45 | - |
3 min, 50% loss of activity | Pseudomonas sp. |
1.14.12.9 | 55 | - |
90-100% loss of activity | Pseudomonas sp. |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.14.12.9 | 7 | 7.5 | - |
Pseudomonas sp. |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
1.14.12.9 | 6 | 7.5 | 4°C, 10 days, 50% loss of activity | Pseudomonas sp. |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.12.9 | FMN | flavoprotein, contains 1 mol of FMN per mol of reductase, no increase of activity by addition of exogenous FMN | Pseudomonas sp. | |
1.14.12.9 | NADH | - |
Pseudomonas sp. | |
1.14.12.9 | NADPH | 30% as effective as NADH | Pseudomonas sp. |