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Literature summary extracted from
- Gene cloning, sequence analysis, and expression of 2-methyl-3-hydroxypyridine-5-carboxylic acid oxygenase (1997), Proc. Natl. Acad. Sci. USA, 94, 7233-7238.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.14.13.242 | expression in Escherichia coli | Pseudomonas sp. |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.13.242 | 0.065 | - |
5-hydroxynicotinic acid | recombinant enzyme | Pseudomonas sp. |  |
| 1.14.13.242 | 0.068 | - |
5-hydroxynicotinic acid | wild-type enzyme | Pseudomonas sp. | |
| 1.14.13.242 | 0.126 | - |
O2 | wild-type enzyme | Pseudomonas sp. | |
| 1.14.13.242 | 0.148 | - |
O2 | recombinant enzyme | Pseudomonas sp. | |
| 1.14.13.242 | 0.18 | - |
NADH | recombinant enzyme | Pseudomonas sp. | |
| 1.14.13.242 | 0.205 | - |
NADH | wild-type enzyme | Pseudomonas sp. | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.14.13.242 | 41700 | - |
4 * 41700, calculation from nucleotide sequence | Pseudomonas sp. |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.13.242 | Pseudomonas sp. | O08453 | MA-1 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.13.242 | - |
Pseudomonas sp. |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.13.242 | 3-hydroxy-2-methylpyridine-5-carboxylate + NADH + O2 | - |
Pseudomonas sp. | 2-(acetamidomethylene)succinate + NAD(P)+ | - |
? | |
| 1.14.13.242 | 5-hydroxynicotinic acid + NADH + O2 | - |
Pseudomonas sp. | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.14.13.242 | tetramer | 4 * 41700, calculation from nucleotide sequence | Pseudomonas sp. |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.13.242 | additional information | - |
additional information | - |
Pseudomonas sp. |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.13.242 | FAD | contains one FAD per subunit, tetrameric enzyme | Pseudomonas sp. | |
| 1.14.13.242 | NADH | - |
Pseudomonas sp. | |
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