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Literature summary extracted from

  • Riedel, A.; Fetzner, S.; Rampp, M.; Lingens, F.; Liebl, U.; Zimmermann, J.L.; Nitschke, W.
    EPR, electron spin echo envelope modulation, and electron nuclear double resonance studies of the 2Fe2S centers of the 2-halobenzoate 1,2-dioxygenase from Burkholderia (Pseudomonas) cepacia 2CBS (1995), J. Biol. Chem., 270, 30869-30873.
    View publication on PubMed

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.12.13 Fe2+ iron atoms are coordinated by 4 cysteines and are bridged by a pair of acid-labile sulfur atoms in the 2Fe2S ferredoxins Burkholderia cepacia
1.14.12.13 Sulfide iron atoms are coordinated by 4 cysteines and are bridged by a pair of acid-labile sulfur atoms in the 2Fe2S ferredoxins Burkholderia cepacia

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.12.13 2-chlorobenzoate + NADH + H+ + O2 Burkholderia cepacia
-
 catechol + chloride + NAD+ + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.12.13 Burkholderia cepacia
-
 Burkholderia cepacia 2CBS, DSM 9959
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.12.13 gel filtration of oxygenase component Burkholderia cepacia

Reaction

EC Number Reaction Comment Organism Reaction ID
1.14.12.13 2-chlorobenzoate + NADH + H+ + O2 = catechol + chloride + NAD+ + CO2 EPR, electron spin echo envelope modulation, electron nuclear double resonance studies and the electrochemical potential are performed. Enzyme contains one ferredoxin-type (reductase) and one Rieske-type (oxygenase) 2Fe2S center Burkholderia cepacia
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.12.13 2-chlorobenzoate + NADH + H+ + O2
-
 Burkholderia cepacia catechol + chloride + NAD+ + CO2
-
 ?