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Literature summary extracted from

  • Ensley, B.D.; Gibson, D.T.; Laborde, A.L.
    Oxidation of naphthalene by a multicomponent enzyme system from Pseudomonas sp. strain NCIB 9816 (1982), J. Bacteriol., 149, 948-954.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
1.14.12.12 10% ethanol and 10% glycerol are required for stability Pseudomonas sp.
1.14.12.12 DTT stabilizes Pseudomonas sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.12.12 cytoplasm
-
 Pseudomonas sp. 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.12.12 Fe2+
-
 Pseudomonas sp.
1.14.12.12 additional information no significant activation by the addition of Fe2+, Fe3+, Zn2+, Mg2+ or Cu2+ Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.12.12 naphthalene + NADH + O2 Pseudomonas sp. initial oxidative reaction in bacterial naphthalene catabolism (1R,2S)-1,2-dihydronaphthalene-1,2-diol + NAD+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.12.12 Pseudomonas sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.12.12 affinity chromatography separates the reductase from the two other enzyme components Pseudomonas sp.
1.14.12.12 purification of ferredoxinNAP reductase, ferredoxinNAP and ISPNAP Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.12.12 naphthalene + NAD(P)H + O2 50% activity with NADH compared to NADPH Pseudomonas sp. cis-(1R,2S)-1,2-dihydronaphthalene-1,2-diol + NAD(P)+
-
 ?
1.14.12.12 naphthalene + NADH + O2
-
 Pseudomonas sp. cis-(1R,2S)-1,2-dihydronaphthalene-1,2-diol + NAD+
-
 ?
1.14.12.12 naphthalene + NADH + O2 initial oxidative reaction in bacterial naphthalene catabolism Pseudomonas sp. (1R,2S)-1,2-dihydronaphthalene-1,2-diol + NAD+
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.12.12 FAD stimulates cytochrome c reduction by ferredoxinNAP reductase Pseudomonas sp.
1.14.12.12 FMN stimulates cytochrome c reduction by ferredoxinNAP reductase Pseudomonas sp.
1.14.12.12 NADH
-
 Pseudomonas sp.
1.14.12.12 NADPH less than 50% of the activity with NADH Pseudomonas sp.