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Literature summary extracted from

  • Armstrong, L.C.; Last, J.A.
    Rat lysyl hydroxylase: molecular cloning, mRNA distribution and expression in a baculovirus system (1995), Biochim. Biophys. Acta, 1264, 93-102.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.14.11.4 medicine deficiency in enzyme activity causes the Ehler-Danlos syndrome type 6 Homo sapiens
1.14.11.4 medicine deficiency in enzyme activity causes the Ehler-Danlos syndrome type 6 Rattus norvegicus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.11.4 expression in insect cells using a baculovirus vector Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.11.4 0.1
-
Procollagen L-lysine
-
Rattus norvegicus
1.14.11.4 0.106
-
2-oxoglutarate
-
Rattus norvegicus
1.14.11.4 0.122
-
ascorbate
-
Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.11.4 85000
-
x * 85000, SDS-PAGE and Western blotting Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
1.14.11.4 Gallus gallus
-
-
-
1.14.11.4 Homo sapiens
-
-
-
1.14.11.4 Rattus norvegicus Q63321
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.11.4
-
Gallus gallus
1.14.11.4
-
Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.11.4 lung
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.11.4 additional information
-
-
Homo sapiens
1.14.11.4 additional information
-
specific activity of the recombinant enzyme three-fold higher than the specific activity of native enzyme Gallus gallus
1.14.11.4 additional information
-
specific activity of the recombinant enzyme three-fold higher than the specific activity of native enzyme Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.11.4 2-oxoglutarate + O2 + ascorbate
-
Rattus norvegicus succinate + CO2 + dehydroascorbate + H2O
-
?
1.14.11.4 peptidyl-L-lysine + 2-oxoglutarate + O2
-
Gallus gallus peptidyl-5-hydroxy-L-lysine + succinate + CO2 hydroxy-L-lysine ?
1.14.11.4 peptidyl-L-lysine + 2-oxoglutarate + O2
-
Homo sapiens peptidyl-5-hydroxy-L-lysine + succinate + CO2 hydroxy-L-lysine ?
1.14.11.4 peptidyl-L-lysine + 2-oxoglutarate + O2
-
Rattus norvegicus peptidyl-5-hydroxy-L-lysine + succinate + CO2 hydroxy-L-lysine ?
1.14.11.4 [procollagen] L-lysine + 2-oxoglutarate + O2
-
Rattus norvegicus [procollagen]-(2S,5R)-5-hydroxy-L-lysine + succinate + CO2
-
r

Subunits

EC Number Subunits Comment Organism
1.14.11.4 ? x * 85000, SDS-PAGE and Western blotting Rattus norvegicus