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Literature summary extracted from

  • Majamaa, K.; Gunzler, V.; Hanauske-Abel, H.M.; Myllylä, R.; Kivirikko, K.I.
    Partial identity of the 2-oxoglutarate and ascorbate binding sites of prolyl 4-hydroxylase (1986), J. Biol. Chem., 261, 7819-7823.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.11.2 bovine serum albumin activation Gallus gallus
1.14.11.2 catalase activation Gallus gallus
1.14.11.2 dithiothreitol activation Gallus gallus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.11.2 (+)-mandelate
-
 Gallus gallus
1.14.11.2 (-)-mandelate
-
 Gallus gallus
1.14.11.2 1,2,3-trihydroxybenzene
-
 Gallus gallus
1.14.11.2 1,2-dihydroxybenzene competitive with respect to 2-oxoglutarate and ascorbate, noncompetitive with respect to Fe2+ Gallus gallus
1.14.11.2 1,3-dihydroxybenzene competitive with respect to 2-oxoglutarate and ascorbate, noncompetitive with respect to Fe2+ Gallus gallus
1.14.11.2 1,4-dihydroxybenzene
-
 Gallus gallus
1.14.11.2 2,3-Dihydroxybenzoate competitive with respect to 2-oxoglutarate and ascorbate, noncompetitive with respect to Fe2+ Gallus gallus
1.14.11.2 2,4-Dihydroxybenzoate competitive with respect to 2-oxoglutarate and ascorbate, noncompetitive with respect to Fe2+ Gallus gallus
1.14.11.2 2,5-Dihydroxybenzoate competitive with respect to 2-oxoglutarate and ascorbate, noncompetitive with respect to Fe2+ Gallus gallus
1.14.11.2 2,6-dihydroxybenzoate competitive with respect to 2-oxoglutarate and ascorbate, noncompetitive with respect to Fe2+ Gallus gallus
1.14.11.2 2-Hydroxybenzoate competitive with respect to 2-oxoglutarate and ascorbate, noncompetitive with respect to Fe2+ Gallus gallus
1.14.11.2 3,4,5-Trihydroxybenzoate
-
 Gallus gallus
1.14.11.2 3,4-dihydroxybenzoate competitive with respect to 2-oxoglutarate and ascorbate, noncompetitive with respect to Fe2+ Gallus gallus
1.14.11.2 3,4-dihydroxycinnamate competitive with respect to 2-oxoglutarate and ascorbate Gallus gallus
1.14.11.2 3,4-dihydroxymandelate competitive with respect to 2-oxoglutarate and ascorbate and noncompetitive with respect to Fe2+ Gallus gallus
1.14.11.2 3,4-Dihydroxyphenylacetate competitive with respect to 2-oxoglutarate and ascorbate, noncompetitive with respect to Fe2+ Gallus gallus
1.14.11.2 3,4-Dihydroxyphenylpropionate competitive with respect to 2-oxoglutarate and ascorbate Gallus gallus
1.14.11.2 3,5-Dihydroxybenzoate competitive with respect to 2-oxoglutarate and ascorbate, noncompetitive with respect to Fe2+ Gallus gallus
1.14.11.2 3-hydroxybenzoate
-
 Gallus gallus
1.14.11.2 4-hydroxybenzoate competitive with respect to 2-oxoglutarate, and noncompetitive with respect to ascorbate Gallus gallus
1.14.11.2 Benzene 1,4-dicarboxylate competitive inhibition with respect to ascorbate Gallus gallus
1.14.11.2 L-galactono gamma-lactone
-
 Gallus gallus
1.14.11.2 phenylacetate
-
 Gallus gallus
1.14.11.2 Pyridine 2,4-dicarboxylate uncompetitive inhibition with respect to ascorbate Gallus gallus
1.14.11.2 Pyridine 2,5-dicarboxylate uncompetitive inhibition with respect to ascorbate Gallus gallus
1.14.11.2 pyridine 2-carboxylate uncompetitive inhibition with respect to ascorbate Gallus gallus

Organism

EC Number Organism UniProt Comment Textmining
1.14.11.2 Gallus gallus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.11.2 embryo
-
 Gallus gallus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.11.2 (Pro-Pro-Gly)n + 2-oxoglutarate + O2 n: 1,5,10 Gallus gallus (Pro-4-hydroxy-Pro-Gly)n + succinate + CO2 n: 1,5,10 ?
1.14.11.2 additional information thermal denaturing of the triple-helical conformation of the substrate before hydroxylation Gallus gallus ?
-
 ?
1.14.11.2 protocollagen + 2-oxoglutarate + O2
-
 Gallus gallus 4-hydroxyproline containing protocollagen + succinate + CO2
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.11.2 37
-
 assay at Gallus gallus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.11.2 7.8
-
 assay at Gallus gallus

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.11.2 5,6-Isopropylideneascorbate can replace ascorbate Gallus gallus
1.14.11.2 ascorbate
-
 Gallus gallus
1.14.11.2 D-isoascorbate can replace ascorbate Gallus gallus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.14.11.2 0.005
-
 3,4-dihydroxybenzoate
-
 Gallus gallus
1.14.11.2 0.009
-
 3,4-Dihydroxyphenylacetate
-
 Gallus gallus
1.14.11.2 0.009
-
 3,4-dihydroxymandelate
-
 Gallus gallus
1.14.11.2 0.013
-
 3,4-Dihydroxyphenylpropionate
-
 Gallus gallus
1.14.11.2 0.018
-
 3,4-dihydroxycinnamate
-
 Gallus gallus
1.14.11.2 0.025
-
 1,2-dihydroxybenzene
-
 Gallus gallus
1.14.11.2 0.03
-
 3,4,5-Trihydroxybenzoate
-
 Gallus gallus
1.14.11.2 0.03
-
 1,2,3-trihydroxybenzene
-
 Gallus gallus
1.14.11.2 0.07
-
 2,3-Dihydroxybenzoate
-
 Gallus gallus
1.14.11.2 0.53
-
 2,4-Dihydroxybenzoate
-
 Gallus gallus
1.14.11.2 1.1
-
 2,6-dihydroxybenzoate
-
 Gallus gallus
1.14.11.2 1.4
-
 2,5-Dihydroxybenzoate
-
 Gallus gallus
1.14.11.2 2.3
-
 2-Hydroxybenzoate
-
 Gallus gallus
1.14.11.2 3.2
-
 4-hydroxybenzoate
-
 Gallus gallus
1.14.11.2 9
-
 3,5-Dihydroxybenzoate
-
 Gallus gallus
1.14.11.2 10.2
-
 1,3-dihydroxybenzene
-
 Gallus gallus
1.14.11.2 14
-
 1,4-dihydroxybenzene
-
 Gallus gallus
1.14.11.2 20
-
 phenylacetate value above Gallus gallus
1.14.11.2 20
-
 3-hydroxybenzoate value above Gallus gallus
1.14.11.2 20
-
 (-)-mandelate value above Gallus gallus
1.14.11.2 20
-
 L-galactono gamma-lactone value above Gallus gallus
1.14.11.2 20
-
 (+)-mandelate value above Gallus gallus