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Literature summary extracted from

  • Tuderman, L.; Myllylä, R.; Kivirikko, K.I.
    Mechanism of the prolyl hydroxylase reaction. 1. Role of co-substrates (1977), Eur. J. Biochem., 80, 341-348.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.11.2 bovine serum albumin activation Gallus gallus
1.14.11.2 catalase activation Gallus gallus
1.14.11.2 dithiothreitol activation Gallus gallus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.11.2 2-oxosuccinate i.e. oxaloacetate Gallus gallus
1.14.11.2 3-hydroxybutyrate less than 10% inhibition Gallus gallus
1.14.11.2 ascorbate at high concentrations Gallus gallus
1.14.11.2 citrate competitive inhibition with respect to 2-oxoglutarate Gallus gallus
1.14.11.2 epinephrine competitive inhibition with respect to Fe2+ Gallus gallus
1.14.11.2 Fe2+ inhibitory at high concentrations Gallus gallus
1.14.11.2 fumarate competitive inhibition with respect to 2-oxoglutarate Gallus gallus
1.14.11.2 isocitrate competitive inhibition with respect to 2-oxoglutarate Gallus gallus
1.14.11.2 Lactate less than 10% inhibition Gallus gallus
1.14.11.2 malate competitive inhibition with respect to 2-oxoglutarate Gallus gallus
1.14.11.2 nitroblue tetrazolium competitive inhibition with respect to O2 Gallus gallus
1.14.11.2 oxaloacetate competitive inhibition with respect to 2-oxoglutarate Gallus gallus
1.14.11.2 poly(L-proline)
-
 Gallus gallus
1.14.11.2 pyruvate competitive inhibition with respect to 2-oxoglutarate Gallus gallus
1.14.11.2 Zn2+ competitive inhibition with respect to Fe2+, non-competitive with respect to the polypeptide substrate and 2-oxoglutarate Gallus gallus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.11.2 Fe2+ absolute requirement, Km: 0.005 mM Gallus gallus

Organism

EC Number Organism UniProt Comment Textmining
1.14.11.2 Gallus gallus
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.14.11.2 procollagen L-proline + 2-oxoglutarate + O2 = procollagen trans-4-hydroxy-L-proline + succinate + CO2 mechanism, role of cosubstrates Gallus gallus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.11.2 embryo
-
 Gallus gallus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.11.2 (Pro-Pro-Gly)n + 2-oxoglutarate + O2 n: 1,5,10 Gallus gallus (Pro-4-hydroxy-Pro-Gly)n + succinate + CO2 n: 1,5,10 ?
1.14.11.2 2-oxoglutarate + O2 + ascorbate uncoupled oxidative decarboxylation Gallus gallus succinate + dehydroascorbate + CO2 + H2O
-
 ?
1.14.11.2 additional information thermal denaturing of the triple-helical conformation of the substrate before hydroxylation Gallus gallus ?
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.11.2 ascorbate Km: 0.2 mM Gallus gallus
1.14.11.2 ascorbate requirement Gallus gallus
1.14.11.2 additional information the ascorbate requirement cannot be replaced by tetrahydrofolic acid, dithiothreitol, NADH2 or dithionite to any significant extent Gallus gallus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.14.11.2 0.0006
-
 Zn2+
-
 Gallus gallus
1.14.11.2 0.04
-
 nitroblue tetrazolium
-
 Gallus gallus
1.14.11.2 0.1
-
 epinephrine
-
 Gallus gallus
1.14.11.2 0.4 0.5 citrate calculated from the slopes or intercepts Gallus gallus
1.14.11.2 0.5 0.6 fumarate calculated from the slopes or intercepts Gallus gallus
1.14.11.2 0.6
-
 Fe2+
-
 Gallus gallus
1.14.11.2 0.6
-
 oxaloacetate
-
 Gallus gallus
1.14.11.2 1.2 1.3 malate calculated from the slopes or intercepts Gallus gallus
1.14.11.2 1.5 2.1 isocitrate calculated from the slopes or intercepts Gallus gallus
1.14.11.2 7
-
 ascorbate
-
 Gallus gallus
1.14.11.2 15 18 pyruvate calculated from the slopes or intercepts Gallus gallus