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Literature summary extracted from

  • Chen, Y.P.; Lovell, C.R.
    Purification and properties of a homodimeric protocatechuate 4,5-dioxygenase from Rhizobium leguminosarum (1994), Arch. Microbiol., 161, 191-195.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.8 0.02
-
 protocatechuate
-
 Rhizobium leguminosarum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.8 Fe2+ 1 mol iron per mol of holoenzyme Rhizobium leguminosarum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.8 62000
-
 2 * 62000, SDS-PAGE Rhizobium leguminosarum
1.13.11.8 120000
-
 gel filtration Rhizobium leguminosarum

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.8 Rhizobium leguminosarum
-
 biovar viceae, strain USDA 2370, grown on 4-hydroxybenzoate-containing medium
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.8
-
 Rhizobium leguminosarum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.8 321
-
-
 Rhizobium leguminosarum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.8 protocatechuate + O2
-
 Rhizobium leguminosarum 4-carboxy-2-hydroxymuconate semialdehyde
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.11.8 homodimer 2 * 62000, SDS-PAGE Rhizobium leguminosarum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.8 9.5 9.8 CAPS Rhizobium leguminosarum