Literature summary extracted from
Fernandez-Canon, J.M.; Penalva, M.A.
Spectrophotometric determination of homogentisate using Aspergillus nidulans homogentisate dioxygenase (1997), Anal. Biochem., 245, 218-221.
Activating Compound
EC Number |
Activating Compound |
Comment |
Organism |
Structure |
---|
1.13.11.5 |
ascorbate |
dependent on the presence of ascorbate, presumably to maintain iron in the reduced form |
Aspergillus nidulans |
|
1.13.11.5 |
additional information |
enzymes of phenylalanine catabolism including HGO are strongly induced by the presence of phenylalanine or phenylacetate |
Aspergillus nidulans |
|
Application
EC Number |
Application |
Comment |
Organism |
---|
1.13.11.5 |
diagnostics |
crude enzyme preparations can be used for a spectrophotometric method for routine, sensitive determination of homogentisate in human urine |
Aspergillus nidulans |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
1.13.11.5 |
additional information |
strain carrying an homogentisate dioxygenase gene disruption with full genotype biA1, methG1, deltahmgA without enzyme activity |
Aspergillus nidulans |
General Stability
EC Number |
General Stability |
Organism |
---|
1.13.11.5 |
rather stable enzyme, can be repeatedly thawed and frozen without significant loss of activity |
Aspergillus nidulans |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
1.13.11.5 |
3,4-Dihydroxyphenylacetate |
1 mM, 48% inhibition, substrate analogue |
Aspergillus nidulans |
|
1.13.11.5 |
p-hydroxyphenylpyruvate |
1 mM, 68% inhibition, substrate analogue |
Aspergillus nidulans |
|
KM Value [mM]
EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Comment |
Organism |
Structure |
---|
1.13.11.5 |
0.009 |
- |
homogentisate |
- |
Aspergillus nidulans |
|
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
1.13.11.5 |
Fe2+ |
dependent on the presence of Fe2+ |
Aspergillus nidulans |
|
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
1.13.11.5 |
homogentisate + O2 |
Aspergillus nidulans |
one of the enzymes mediating phenylalanine catabolism |
4-maleylacetoacetate |
- |
? |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
1.13.11.5 |
Aspergillus nidulans |
- |
filamentous ascomycete |
- |
1.13.11.5 |
Aspergillus nidulans |
- |
strains biA1 and veA1 |
- |
Specific Activity [micromol/min/mg]
EC Number |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Comment |
Organism |
---|
1.13.11.5 |
0.75 |
1 |
value in crude lysates |
Aspergillus nidulans |
Storage Stability
EC Number |
Storage Stability |
Organism |
---|
1.13.11.5 |
-20°C, 50 mM potassium phosphate buffer, pH 7.0, crude extracts, 5 months, 25% loss of activity |
Aspergillus nidulans |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
1.13.11.5 |
homogentisate + O2 |
highly specific for homogentisate |
Aspergillus nidulans |
4-maleylacetoacetate |
- |
? |
|
1.13.11.5 |
homogentisate + O2 |
one of the enzymes mediating phenylalanine catabolism |
Aspergillus nidulans |
4-maleylacetoacetate |
- |
? |
|
1.13.11.5 |
additional information |
not as substrates: phenylacetate, 2-hydroxyphenylacetate, 3-hydroxyphenylacetate, 4-hydroxyphenylacetate, phenylalanine, tyrosine, phenylpyruvate, gentisate |
Aspergillus nidulans |
? |
- |
? |
|
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
1.13.11.5 |
6.5 |
7 |
- |
Aspergillus nidulans |