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Literature summary extracted from

  • Latus, M.; Seitz, H.J.; Eberspaecher, J.; Lingens, F.
    Purification and characterization of hydroxyquinol 1,2-dioxygenase from Azotobacter sp. strain GP1 (1995), Appl. Environ. Microbiol., 61, 2453-2460.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.13.11.37 Fe(NH4)2(SO4)2 0.00001-0.000001 mM Azotobacter sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.37 Tiron
-
 Azotobacter sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.37 Iron 0.18 mol per mol enzyme Azotobacter sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.37 34250
-
 2 * 34250, SDS-PAGE Azotobacter sp.
1.13.11.37 58000
-
 gel filtration Azotobacter sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.37 benzene-1,2,4-triol + O2 Azotobacter sp.
-
 3-hydroxy-cis-cis-muconate
-
 ?
1.13.11.37 benzene-1,2,4-triol + O2 Azotobacter sp. GP1 / DSM 6428 / ATCC 49806
-
 3-hydroxy-cis-cis-muconate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.37 Azotobacter sp.
-
 grown with 2,4,6-trichlorophenol
-
1.13.11.37 Azotobacter sp. GP1 / DSM 6428 / ATCC 49806
-
 grown with 2,4,6-trichlorophenol
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.37
-
 Azotobacter sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.37 7.06
-
 reactivated with 0.01 mM Fe2+ Azotobacter sp.

Storage Stability

EC Number Storage Stability Organism
1.13.11.37 -80°C, no loss of activity after several weeks, at room temperature no considerable decrease after 1 day Azotobacter sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.37 6-chlorohydroxyquinol + O2 33.5% relative activity of that measured with hydroxyquinol Azotobacter sp. 2-chloromaleylacetate
-
 ?
1.13.11.37 6-chlorohydroxyquinol + O2 33.5% relative activity of that measured with hydroxyquinol Azotobacter sp. GP1 / DSM 6428 / ATCC 49806 2-chloromaleylacetate
-
 ?
1.13.11.37 benzene-1,2,4-triol + O2
-
 Azotobacter sp. maleylacetate maleylacetate, i.e. cis-hexenedioate, is the isomer of 3-hydroxy-cis-cis-muconate ?
1.13.11.37 benzene-1,2,4-triol + O2
-
 Azotobacter sp. GP1 / DSM 6428 / ATCC 49806 maleylacetate maleylacetate, i.e. cis-hexenedioate, is the isomer of 3-hydroxy-cis-cis-muconate ?
1.13.11.37 benzene-1,2,4-triol + O2
-
 Azotobacter sp. 3-hydroxy-cis-cis-muconate
-
 ?
1.13.11.37 benzene-1,2,4-triol + O2
-
 Azotobacter sp. GP1 / DSM 6428 / ATCC 49806 3-hydroxy-cis-cis-muconate
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.11.37 dimer 2 * 34250, SDS-PAGE Azotobacter sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.37 6.4
-
 in succinate buffer Azotobacter sp.