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Literature summary extracted from

  • Muller, R.; Haug, S.; Eberspächer, J.; Lingens, F.
    Catechol 2,3-dioxygenase from pyrazon-degrading bacteria (1977), Hoppe-Seyler's Z. Physiol. Chem., 358, 797-805.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.13.11.36 high osmotic sensitivity Phenylobacterium immobile

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.36 high ionic strength
-
 Phenylobacterium immobile

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.36 0.017
-
 3-Methylpyrocatechol
-
 Phenylobacterium immobile
1.13.11.36 0.16
-
 4-Methylpyrocatechol
-
 Phenylobacterium immobile
1.13.11.36 0.2
-
 5-Amino-4-chloro-2-(2,3-dihydroxyphenyl)-3(2H)-pyridazinone
-
 Phenylobacterium immobile
1.13.11.36 0.66
-
 pyrocatechol
-
 Phenylobacterium immobile

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.36 Co2+ can partially replace Fe2+ Phenylobacterium immobile
1.13.11.36 Fe2+ required for activity Phenylobacterium immobile
1.13.11.36 Zn2+ can partially replace Fe2+ Phenylobacterium immobile

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.36 34000
-
 6 * 34000, SDS-PAGE Phenylobacterium immobile
1.13.11.36 200000
-
 analytical ultracentrifugation Phenylobacterium immobile
1.13.11.36 220000
-
 gel filtration Phenylobacterium immobile

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.36 5-amino-4-chloro-2-(2,3-dihydroxyphenyl)-3(2H)-pyridazinone + O2 Phenylobacterium immobile enzyme of pyrazone metabolism 5-amino-4-chloro-2-(2-hydroxymuconoyl)-3(2H)-pyridazinone
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.36 Phenylobacterium immobile
-
 DSM 1986
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.36
-
 Phenylobacterium immobile

Storage Stability

EC Number Storage Stability Organism
1.13.11.36 -20°C, stable for months Phenylobacterium immobile

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.36 3-methylpyrocatechol + O2
-
 Phenylobacterium immobile 2-hydroxy-3-methylmuconic acid semialdehyde
-
 ?
1.13.11.36 4-methylpyrocatechol + O2
-
 Phenylobacterium immobile 2-hydroxy-3-methylmuconic acid semialdehyde
-
 ?
1.13.11.36 5-amino-4-chloro-2-(2,3-dihydroxyphenyl)-3(2H)-pyridazinone + O2
-
 Phenylobacterium immobile 5-amino-4-chloro-2-(2-hydroxymuconoyl)-3(2H)-pyridazinone
-
 ?
1.13.11.36 5-amino-4-chloro-2-(2,3-dihydroxyphenyl)-3(2H)-pyridazinone + O2 enzyme of pyrazone metabolism Phenylobacterium immobile 5-amino-4-chloro-2-(2-hydroxymuconoyl)-3(2H)-pyridazinone
-
 ?
1.13.11.36 catechol + O2
-
 Phenylobacterium immobile 2-hydroxymuconic acid semialdehyde
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.11.36 hexamer 6 * 34000, SDS-PAGE Phenylobacterium immobile

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.11.36 30
-
-
 Phenylobacterium immobile

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.36 7 8
-
 Phenylobacterium immobile