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Literature summary extracted from

  • Kataeva, I.A.; Golovleva, L.A.
    Catechol 2,3-dioxygenases from Pseudomonas aeruginosa 2x (1990), Methods Enzymol., 188, 115-121.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.2 Ag+
-
 Pseudomonas aeruginosa
1.13.11.2 CuCl
-
 Pseudomonas aeruginosa
1.13.11.2 CuSO4
-
 Pseudomonas aeruginosa
1.13.11.2 o-phenanthroline
-
 Pseudomonas aeruginosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.2 0.002
-
 catechol metapyrocatechase 2 Pseudomonas aeruginosa
1.13.11.2 0.0041
-
 catechol metapyrocatechase 1 Pseudomonas aeruginosa
1.13.11.2 0.008
-
 4-methylcatechol metapyrocatechase 2 Pseudomonas aeruginosa
1.13.11.2 0.01
-
 3-methylcatechol metapyrocatechase 2 Pseudomonas aeruginosa
1.13.11.2 0.02
-
 4-methylcatechol metapyrocatechase 1 Pseudomonas aeruginosa
1.13.11.2 0.06
-
 3-methylcatechol metapyrocatechase 1 Pseudomonas aeruginosa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.2 Fe2+ FeCl2 and FeSO4 activate metapyrocatechase 2, no effect on metapyrocatechase 1 Pseudomonas aeruginosa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.2 33500
-
 4 * 33500 metapyrocatechase 1, SDS-PAGE Pseudomonas aeruginosa
1.13.11.2 36000
-
 4 * 36000, metapyrocatechase 2, SDS-PAGE Pseudomonas aeruginosa
1.13.11.2 132000 134000 gel filtration, sedimentation equilibrium analysis, amino acid analysis, metapyrocatechase 2 Pseudomonas aeruginosa
1.13.11.2 143000 144600 gel filtration, sedimentation equilibrium analysis, amino acid analysis Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.2 catechol + O2 Pseudomonas aeruginosa metapyrocatechase 1 takes part in oxidation of aromatic compounds, producing catechol as an intermediate, metapyrocatechase 2 is involved in oxidation of methyl-substituted aromatic substrates 2-hydroxymuconate semialdehyde
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.2 Pseudomonas aeruginosa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.2 metapyrocatechase 1 and 2 Pseudomonas aeruginosa

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.2 35
-
 metapyrocatechase 2 Pseudomonas aeruginosa
1.13.11.2 90
-
 metapyrocatechase 1 Pseudomonas aeruginosa

Storage Stability

EC Number Storage Stability Organism
1.13.11.2 -20°C, in lyophilized state for several months, metapyrocatechase 1 and 2 Pseudomonas aeruginosa
1.13.11.2 4°C, in acetone buffer under nitrogen for several weeks, metapyrocatechase 1 and 2 Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.2 3,4-dihydroxybenzoate + O2 weak Pseudomonas aeruginosa ?
-
 ?
1.13.11.2 3-chlorocatechol + O2 weak Pseudomonas aeruginosa 2-chloro-2-hydroxy-6-oxohexa-2,4-dienoate
-
 ?
1.13.11.2 3-methylcatechol + O2 weak Pseudomonas aeruginosa 2-hydroxy-6-oxohepta-2,4-dienoate
-
 ?
1.13.11.2 4-methylcatechol + O2
-
 Pseudomonas aeruginosa 2-hydroxy-3-methyl-6-oxohexa-2,4-dienoate
-
 ?
1.13.11.2 catechol + O2
-
 Pseudomonas aeruginosa 2-hydroxymuconate semialdehyde
-
 ?
1.13.11.2 catechol + O2 metapyrocatechase 1 takes part in oxidation of aromatic compounds, producing catechol as an intermediate, metapyrocatechase 2 is involved in oxidation of methyl-substituted aromatic substrates Pseudomonas aeruginosa 2-hydroxymuconate semialdehyde
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.11.2 tetramer 4 * 33500 metapyrocatechase 1, SDS-PAGE Pseudomonas aeruginosa
1.13.11.2 tetramer 4 * 36000, metapyrocatechase 2, SDS-PAGE Pseudomonas aeruginosa