Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Gibello, A.; Ferrer, E.; Martin, M.; Garrido-Pertierra, A.
    3,4-Dihydroxyphenylacetate 2,3-dioxygenase from Klebsiella pneumoniae, a Mg2+-containing dioxygenase involved in aromatic catabolism (1994), Biochem. J., 301, 145-150.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.15 3,4-dichlorophenylacetate 5 mM, 50% inhibition Klebsiella pneumoniae
1.13.11.15 3,4-dihydroxybenzoate 1 mM, 75% inhibition Klebsiella pneumoniae
1.13.11.15 Cu2+ 0.01 mM, 80% inhibition Klebsiella pneumoniae
1.13.11.15 H2O2 10 mM; immediate loss of activity Klebsiella pneumoniae
1.13.11.15 succinate semialdehyde 5 mM 65% inhibition Klebsiella pneumoniae
1.13.11.15 Zn2+ 0.01 mM, complete inhibition Klebsiella pneumoniae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.15 0.074
-
 3,4-dihydroxybenzoic acid
-
 Klebsiella pneumoniae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.15 Iron less than 0.01 gatom Fe2+ per mol of enzyme Klebsiella pneumoniae
1.13.11.15 Mg2+ 1.1-1.3 gatom of Mg2+ per mol of enzyme, essential for activity Klebsiella pneumoniae
1.13.11.15 Mn2+ 0.01-0.3 gatom of Mn2+ per mol of enzyme Klebsiella pneumoniae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.15 25500
-
 4 * 25500, SDS-PAGE Klebsiella pneumoniae
1.13.11.15 102000
-
 gel filtration Klebsiella pneumoniae

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.15 Klebsiella pneumoniae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.15 recombinant enzyme from Escherichia coli Klebsiella pneumoniae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.15 102
-
-
 Klebsiella pneumoniae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.15 3,4-dihydroxybenzoic acid + O2
-
 Klebsiella pneumoniae 2-hydroxy-5-carboxymuconate semialdehyde
-
 ?
1.13.11.15 3,4-dihydroxyphenylacetate + O2
-
 Klebsiella pneumoniae 2-hydroxy-5-carboxymethylmuconate semialdehyde
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.11.15 tetramer 4 * 25500, SDS-PAGE Klebsiella pneumoniae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.11.15 30
-
-
 Klebsiella pneumoniae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.13.11.15
-
-
 pH 7.5, 0.1 M sodium potassium phosphate, 1 mM DTT, complete loss of activity after 48 h Klebsiella pneumoniae
1.13.11.15 40
-
 half-life: 13 min Klebsiella pneumoniae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.15 7.5
-
-
 Klebsiella pneumoniae

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.13.11.15 7.5
-
 maximal stability at Klebsiella pneumoniae