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Literature summary extracted from

  • Fauque, G.; Czechowski, M.; Berlier, Y.M.; Lespinat, P.A.; LeGall, J.; Moura, J.J.G.
    Partial purification and characterization of the first hydrogenase isolated from a thermophilic sulfate-reducing bacterium (1992), Biochem. Biophys. Res. Commun., 184, 1256-1260.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.12.99.6 NO
-
Thermodesulfobacterium thermophilum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.12.99.6 Fe 7-8 atoms per mol enzyme, 3Fe-4S cluster Thermodesulfobacterium thermophilum
1.12.99.6 Ni 0.6-0.7 atoms per mol enzyme Thermodesulfobacterium thermophilum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.12.99.6 15000
-
1 * 55000 + 1 * 15000, SDS-PAGE Thermodesulfobacterium thermophilum
1.12.99.6 55000
-
1 * 55000 + 1 * 15000, SDS-PAGE Thermodesulfobacterium thermophilum

Organism

EC Number Organism UniProt Comment Textmining
1.12.99.6 Thermodesulfobacterium thermophilum
-
strain DSM 1276 formerly Desulfovibrio thermophilus
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.12.99.6 partial Thermodesulfobacterium thermophilum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.12.99.6 783
-
H2 uptake Thermodesulfobacterium thermophilum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.12.99.6 H2 + acceptor benzyl viologen acts as electron acceptor Thermodesulfobacterium thermophilum H+ + reduced acceptor
-
?
1.12.99.6 H2 + acceptor methyl viologen acts as electron acceptor Thermodesulfobacterium thermophilum H+ + reduced acceptor
-
?

Subunits

EC Number Subunits Comment Organism
1.12.99.6 dimer 1 * 55000 + 1 * 15000, SDS-PAGE Thermodesulfobacterium thermophilum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.12.99.6 60
-
-
Thermodesulfobacterium thermophilum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.12.99.6 4.5
-
H2 production Thermodesulfobacterium thermophilum
1.12.99.6 7.8
-
H2 uptake Thermodesulfobacterium thermophilum