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Literature summary extracted from

  • Ma, K.; Weiss, R.; Adams, M.W.W.
    Characterization of hydrogenase II from the hyperthermophilic archaeon Pyrococcus furiosus and assessment of its role in sulfur reduction (2000), J. Bacteriol., 182, 1864-1871.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.12.7.2 0.017
-
 NADP+
-
 Pyrococcus furiosus
1.12.7.2 0.07
-
 NADPH
-
 Pyrococcus furiosus
1.12.7.2 0.07
-
 NADH
-
 Pyrococcus furiosus
1.12.7.2 0.125
-
 NAD+
-
 Pyrococcus furiosus
1.12.7.2 0.2
-
 sulfur
-
 Pyrococcus furiosus
1.12.7.2 1
-
 methyl viologen at 120 kPa H2 Pyrococcus furiosus
1.12.7.2 1.25
-
 reduced methyl viologen
-
 Pyrococcus furiosus
1.12.99.6 additional information
-
 additional information Km for various electron donors Pyrococcus furiosus
1.12.99.6 0.128
-
 NAD+
-
 Pyrococcus furiosus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.12.1.5 cytoplasm
-
 Pyrococcus furiosus 5737
-
1.12.7.2 cytoplasm
-
 Pyrococcus furiosus 5737
-
1.12.98.4 cytoplasm
-
 Pyrococcus furiosus 5737
-
1.12.99.6 cytoplasm
-
 Pyrococcus furiosus 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.12.1.5 Fe2+ contains 21 atoms iron per mol enzyme Pyrococcus furiosus
1.12.1.5 Ni2+ contains 0.9 atoms nickel per mol enzyme Pyrococcus furiosus
1.12.7.2 Fe five [4Fe4S] clusters, 2 in the beta subunit and 3 in the delta subunit, additionally one [2Fe2S] cluster in the gamma subunit Pyrococcus furiosus
1.12.7.2 Fe 21 atoms per mol heterotetramer Pyrococcus furiosus
1.12.7.2 Ni 0.9 atoms per mol heterotetramer, NiFe-catalytic site in the alpha-subunit Pyrococcus furiosus
1.12.98.4 Fe2+ contains 21 atoms iron per mol enzyme Pyrococcus furiosus
1.12.98.4 Ni2+ contains 0.9 atoms nickel per mol enzyme Pyrococcus furiosus
1.12.99.6 Fe 21 atoms per mol enzyme, 5 4Fe-4S cluster and 1 2Fe-2S cluster Pyrococcus furiosus
1.12.99.6 Ni 0.9 atoms per mol enzyme Pyrococcus furiosus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.12.1.5 24000
-
 1 * 52000 + 1 * 39000 + 1 * 30000 + 1 * 24000, SDS-PAGE Pyrococcus furiosus
1.12.1.5 30000
-
 1 * 52000 + 1 * 39000 + 1 * 30000 + 1 * 24000, SDS-PAGE Pyrococcus furiosus
1.12.1.5 39000
-
 1 * 52000 + 1 * 39000 + 1 * 30000 + 1 * 24000, SDS-PAGE Pyrococcus furiosus
1.12.1.5 52000
-
 1 * 52000 + 1 * 39000 + 1 * 30000 + 1 * 24000, SDS-PAGE Pyrococcus furiosus
1.12.1.5 320000
-
 gel filtration Pyrococcus furiosus
1.12.7.2 24000
-
 alpha, beta, gamma, delta, 2 * 52000 + 2 * 39000 + 2 * 30000 + 2 * 24000, SDS-PAGE Pyrococcus furiosus
1.12.7.2 30000
-
 alpha, beta, gamma, delta, 2 * 52000 + 2 * 39000 + 2 * 30000 + 2 * 24000, SDS-PAGE Pyrococcus furiosus
1.12.7.2 39000
-
 alpha, beta, gamma, delta, 2 * 52000 + 2 * 39000 + 2 * 30000 + 2 * 24000, SDS-PAGE Pyrococcus furiosus
1.12.7.2 52000
-
 alpha, beta, gamma, delta, 2 * 52000 + 2 * 39000 + 2 * 30000 + 2 * 24000, SDS-PAGE Pyrococcus furiosus
1.12.7.2 320000
-
 gel filtration Pyrococcus furiosus
1.12.98.4 24000
-
 1 * 52000 + 1 * 39000 + 1 * 30000 + 1 * 24000, SDS-PAGE Pyrococcus furiosus
1.12.98.4 30000
-
 1 * 52000 + 1 * 39000 + 1 * 30000 + 1 * 24000, SDS-PAGE Pyrococcus furiosus
1.12.98.4 39000
-
 1 * 52000 + 1 * 39000 + 1 * 30000 + 1 * 24000, SDS-PAGE Pyrococcus furiosus
1.12.98.4 52000
-
 1 * 52000 + 1 * 39000 + 1 * 30000 + 1 * 24000, SDS-PAGE Pyrococcus furiosus
1.12.98.4 320000
-
 gel filtration Pyrococcus furiosus
1.12.99.6 24000
-
 alpha,beta,gamma,delta, 1 * 52000 + 1 * 39000 + 1 * 30000 + 1 * 24000, SDS-PAGE Pyrococcus furiosus
1.12.99.6 30000
-
 alpha,beta,gamma,delta, 1 * 52000 + 1 * 39000 + 1 * 30000 + 1 * 24000, SDS-PAGE Pyrococcus furiosus
1.12.99.6 39000
-
 alpha,beta,gamma,delta, 1 * 52000 + 1 * 39000 + 1 * 30000 + 1 * 24000, SDS-PAGE Pyrococcus furiosus
1.12.99.6 52000
-
 alpha,beta,gamma,delta, 1 * 52000 + 1 * 39000 + 1 * 30000 + 1 * 24000, SDS-PAGE Pyrococcus furiosus
1.12.99.6 320000
-
 gel filtration Pyrococcus furiosus

Organism

EC Number Organism UniProt Comment Textmining
1.12.1.5 Pyrococcus furiosus
-
-
-
1.12.1.5 Pyrococcus furiosus E7FHC4 subunit alpha
-
1.12.1.5 Pyrococcus furiosus E7FHF8 subunit delta
-
1.12.1.5 Pyrococcus furiosus E7FHN9 subunit beta
-
1.12.1.5 Pyrococcus furiosus E7FHW8 subunit gamma
-
1.12.7.2 Pyrococcus furiosus
-
 strain DSM 3638
-
1.12.7.2 Pyrococcus furiosus
-
 hydrogenase II
-
1.12.98.4 Pyrococcus furiosus E7FHC4 and E7FHN9 and E7FHW8 and E7FHF8 subunits alpha, beta, gamma and delta
-
1.12.99.6 Pyrococcus furiosus
-
 strain DSM 3638
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.12.1.5 DEAE column chromatography, hydroxyapatite column chromatography, phenyl Sepharose column chromatography, and Superdex 200 gel filtration Pyrococcus furiosus
1.12.98.4 DEAE column chromatography, hydroxyapatite column chromatography, phenyl Sepharose column chromatography, and Superdex 200 gel filtration Pyrococcus furiosus
1.12.99.6
-
 Pyrococcus furiosus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.12.7.2 0.02
-
 hydrogen production, oxidized ferredoxin as electron donor, hydrogenase II Pyrococcus furiosus
1.12.7.2 0.2
-
 sulfur reduction, hydrogenase II Pyrococcus furiosus
1.12.7.2 0.98
-
 hydrogen production, sodium dithionite as electron donor, hydrogenase II Pyrococcus furiosus
1.12.7.2 11
-
 hydrogen oxidation, methyl viologen as electron acceptor, hydrogenase II Pyrococcus furiosus
1.12.7.2 40
-
 hydrogen production, methyl viologen as electron donor, hydrogenase II Pyrococcus furiosus
1.12.7.2 131
-
 hydrogen oxidation, benzyl viologen as electron acceptor, hydrogenase II Pyrococcus furiosus
1.12.99.6 additional information
-
 specific activities with various electron donors Pyrococcus furiosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.12.1.5 H2 + NAD+
-
 Pyrococcus furiosus H+ + NADH
-
 r
1.12.1.5 H2 + NADP+
-
 Pyrococcus furiosus H+ + NADPH
-
 r
1.12.1.5 additional information ferredoxin from Pyrococcus furiosus is not an efficient electron carrier Pyrococcus furiosus ?
-
 ?
1.12.7.2 H+ + NADH
-
 Pyrococcus furiosus H2 + NAD+
-
 r
1.12.7.2 H+ + reduced methyl viologen
-
 Pyrococcus furiosus H2 + oxidized methyl viologen
-
 ?
1.12.7.2 H2 + electron acceptor methyl viologen as electron acceptor Pyrococcus furiosus H+ + reduced electron acceptor
-
 r
1.12.7.2 H2 + electron acceptor benzyl viologen as electron acceptor Pyrococcus furiosus H+ + reduced electron acceptor
-
 r
1.12.7.2 H2 + electron acceptor NADP+ as electron acceptor Pyrococcus furiosus H+ + reduced electron acceptor
-
 r
1.12.7.2 H2 + NAD+
-
 Pyrococcus furiosus H+ + NADH
-
 r
1.12.7.2 additional information ferredoxin is not an efficient electron carrier for both hydrogenases Pyrococcus furiosus ?
-
 ?
1.12.7.2 S + NADPH
-
 Pyrococcus furiosus H2S + NADP+
-
 ?
1.12.98.4 H2 + benzyl viologen highest activity Pyrococcus furiosus H2S + reduced benzyl viologen
-
 ?
1.12.98.4 H2 + methyl viologen
-
 Pyrococcus furiosus H2S + reduced methyl viologen
-
 ?
1.12.98.4 H2 + polysulfide(n)
-
 Pyrococcus furiosus H2S + polysulfide(n-1)
-
 r
1.12.98.4 additional information ferredoxin from Pyrococcus furiosus is not an efficient electron carrier Pyrococcus furiosus ?
-
 ?
1.12.98.4 NADPH + H+ + polysulfide(n)
-
 Pyrococcus furiosus NADP+ + polysulfide(n-1)
-
 r
1.12.99.6 H2 + acceptor benzyl viologen, methyl viologen, NADPH and NADH can serve as electron donors Pyrococcus furiosus H+ + reduced acceptor
-
 ?
1.12.99.6 H2 + NAD+
-
 Pyrococcus furiosus NADH + H+
-
 r
1.12.99.6 H2 + S
-
 Pyrococcus furiosus H2S
-
 ?

Subunits

EC Number Subunits Comment Organism
1.12.1.5 heterotetramer 1 * 52000 + 1 * 39000 + 1 * 30000 + 1 * 24000, SDS-PAGE Pyrococcus furiosus
1.12.7.2 octamer alpha, beta, gamma, delta, 2 * 52000 + 2 * 39000 + 2 * 30000 + 2 * 24000, SDS-PAGE Pyrococcus furiosus
1.12.98.4 heterotetramer 1 * 52000 + 1 * 39000 + 1 * 30000 + 1 * 24000, SDS-PAGE Pyrococcus furiosus
1.12.99.6 tetramer alpha,beta,gamma,delta, 1 * 52000 + 1 * 39000 + 1 * 30000 + 1 * 24000, SDS-PAGE Pyrococcus furiosus

Synonyms

EC Number Synonyms Comment Organism
1.12.1.5 H-II
-
 Pyrococcus furiosus
1.12.1.5 hydrogenase II
-
 Pyrococcus furiosus
1.12.98.4 H-II
-
 Pyrococcus furiosus
1.12.98.4 hydrogenase II
-
 Pyrococcus furiosus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.12.7.2 90
-
 above 90°C, hydrogenase II Pyrococcus furiosus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.12.7.2 95
-
 50% inactivation after 6 h, hydrogenase II Pyrococcus furiosus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.12.99.6 additional information
-
 additional information turnover number for various electron donors Pyrococcus furiosus

Cofactor

EC Number Cofactor Comment Organism Structure
1.12.1.5 FAD contains 0.83 FAD per mol enzyme Pyrococcus furiosus
1.12.1.5 NADPH
-
 Pyrococcus furiosus
1.12.1.5 [2Fe-2S]-center contains 1 [2Fe-2S]-center Pyrococcus furiosus
1.12.1.5 [4Fe-4S]-center contains 2 [4Fe-4S]-centers Pyrococcus furiosus
1.12.7.2 FAD putative nucleotide-binding site in the gamma subunit Pyrococcus furiosus
1.12.7.2 FAD hydrogenase II, 0.83 mol per mol heterotetramer Pyrococcus furiosus
1.12.7.2 NADH putative nucleotide-binding site in the gamma subunit Pyrococcus furiosus
1.12.7.2 NADPH putative nucleotide-binding site in the gamma subunit Pyrococcus furiosus
1.12.98.4 FAD contains 0.83 FAD per mol enzyme Pyrococcus furiosus
1.12.98.4 NADH NADPH and NADH are equally efficient as electron donors for H2 production Pyrococcus furiosus
1.12.98.4 NADPH NADPH and NADH are equally efficient as electron donors for H2 production Pyrococcus furiosus
1.12.98.4 [2Fe-2S]-center contains 1 [2Fe-2S] center Pyrococcus furiosus
1.12.98.4 [4Fe-4S]-center contains 2 [4Fe-4S]-centers Pyrococcus furiosus
1.12.99.6 FAD 0.83 mol FAD per enzyme Pyrococcus furiosus