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Literature summary extracted from

  • Serebryakova, L.T.; Medina, M.; Zorin, N.A.; Gogotov, I.N.; Cammack, R.
    Reversible hydrogenase of Anabaena variabilis ATCC 29413. Catalytic properties and characterization of redox centers (1996), FEBS Lett., 383, 79-82.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.12.99.6 50% loss of activity after 10 min in air Trichormus variabilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.12.99.6 CO weak, reversible, competitive Trichormus variabilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.12.99.6 0.0113
-
H2
-
Trichormus variabilis
1.12.99.6 0.055
-
oxidized methyl viologen purified enzyme Trichormus variabilis
1.12.99.6 0.055
-
oxidized methyl viologen purified enzyme, at pH 8.0 and 30°C Trichormus variabilis
1.12.99.6 0.17
-
oxidized methyl viologen cell free extract Trichormus variabilis
1.12.99.6 0.17
-
oxidized methyl viologen cell-free extract, at pH 8.0 and 30°C Trichormus variabilis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.12.99.6 Fe 4Fe-4S and 2Fe-2S clusters Trichormus variabilis
1.12.99.6 Ni
-
Trichormus variabilis

Organism

EC Number Organism UniProt Comment Textmining
1.12.99.6 Trichormus variabilis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.12.99.6
-
Trichormus variabilis

Storage Stability

EC Number Storage Stability Organism
1.12.99.6 4°C, 50% loss of activity in cell free extracts after 7 days, 50% loss of activity of purified enzyme after 26 h Trichormus variabilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.12.99.6 H2 + acceptor methyl viologen and benzyl viologen can serve as electron acceptors Trichormus variabilis H+ + reduced acceptor
-
?
1.12.99.6 H2 + oxidized methyl viologen
-
Trichormus variabilis reduced methyl viologen
-
r

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.12.99.6 6.8
-
-
Trichormus variabilis

Cofactor

EC Number Cofactor Comment Organism Structure
1.12.99.6 FMN
-
Trichormus variabilis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.12.99.6 0.0355
-
CO
-
Trichormus variabilis