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Literature summary extracted from

  • Sawers, R.G.; Boxer, D.H.
    Purification and properties of membrane-bound hydrogenase isoenzyme 1 from anaerobically grown Escherichia coli K12 (1986), Eur. J. Biochem., 156, 265-275.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.12.99.6 90% loss of activity after 8 days in the presence of oxygen, increasing ionic strength causes 10-40% reversible inhibition Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.12.99.6 Cu2+ 95% inhibition at 0.1 mM Escherichia coli
1.12.99.6 N-bromosuccinimide complete inactivation at 10 mM, 20% inactivation at 0.1 mM Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.12.99.6 0.002
-
 H2
-
 Escherichia coli
1.12.99.6 0.0045
-
 benzyl viologen
-
 Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.12.99.6 Fe 12.2 mol per mol enzyme, iron-sulfur protein Escherichia coli
1.12.99.6 Sulfide 9.1 mol per mol enzyme, iron-sulfur protein Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.12.99.6 29000
-
 1 * 64000, 1 * 31000, 1 * 29000, SDS-PAGE Escherichia coli
1.12.99.6 31000
-
 1 * 64000, 1 * 31000, 1 * 29000, SDS-PAGE Escherichia coli
1.12.99.6 64000
-
 1 * 64000, 1 * 31000, 1 * 29000, SDS-PAGE Escherichia coli
1.12.99.6 200000
-
 gel filtration Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
1.12.99.6 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.12.99.6
-
 Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.12.99.6 10.6
-
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.12.99.6 H2 + acceptor benzyl viologen, methylene blue and methylviologen can act as electron acceptors Escherichia coli H+ + reduced acceptor
-
 ?
1.12.99.6 H2 + oxidized benzyl viologen
-
 Escherichia coli H+ + reduced benzyl viologen
-
 ?

Subunits

EC Number Subunits Comment Organism
1.12.99.6 trimer 1 * 64000, 1 * 31000, 1 * 29000, SDS-PAGE Escherichia coli

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.12.99.6 4 10 enzyme is still 60% active after 4 h at pH 4 or pH 10 Escherichia coli