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Literature summary extracted from

  • Fischer, J.; Quentmeier, A.; Kostka, S.; Kraft, R.; Friedrich, C.G.
    Purification and characterization of the hydrogenase from Thiobacillus ferrooxidans (1996), Arch. Microbiol., 165, 289-296.
    View publication on PubMed

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.12.7.2 Fe 6.02 mol per mol enzyme Acidithiobacillus ferrooxidans
1.12.7.2 Ni 0.72 mol per mol enzyme Acidithiobacillus ferrooxidans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.12.7.2 34000
-
 1 * 64000 + 1 * 34000, SDS-PAGE Acidithiobacillus ferrooxidans
1.12.7.2 64000
-
 1 * 64000 + 1 * 34000, SDS-PAGE Acidithiobacillus ferrooxidans
1.12.7.2 100000
-
 native PAGE Acidithiobacillus ferrooxidans

Organism

EC Number Organism UniProt Comment Textmining
1.12.7.2 Acidithiobacillus ferrooxidans
-
-
-
1.12.7.2 Acidithiobacillus ferrooxidans ATC 19859
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.12.7.2
-
 Acidithiobacillus ferrooxidans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.12.7.2 6.02
-
 hydrogen oxidation, methylene blue as electron acceptor Acidithiobacillus ferrooxidans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.12.7.2 H2 + electron acceptor ferricyanide as electron acceptor Acidithiobacillus ferrooxidans H+ + reduced electron acceptor
-
 r
1.12.7.2 H2 + electron acceptor methylene blue as electron acceptor Acidithiobacillus ferrooxidans H+ + reduced electron acceptor
-
 r
1.12.7.2 H2 + electron acceptor ferricyanide as electron acceptor Acidithiobacillus ferrooxidans ATC 19859 H+ + reduced electron acceptor
-
 r
1.12.7.2 H2 + electron acceptor methylene blue as electron acceptor Acidithiobacillus ferrooxidans ATC 19859 H+ + reduced electron acceptor
-
 r

Subunits

EC Number Subunits Comment Organism
1.12.7.2 dimer 1 * 64000 + 1 * 34000, SDS-PAGE Acidithiobacillus ferrooxidans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.12.7.2 49
-
-
 Acidithiobacillus ferrooxidans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.12.7.2 9
-
-
 Acidithiobacillus ferrooxidans