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Literature summary extracted from

  • Roessler, P.G.; Lien, S.
    Purification of hydrogenase from Chlamydomonas reinhardtii (1984), Plant Physiol., 75, 705-709.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.12.7.2 0.01
-
 Ferredoxin hydrogen evolution Chlamydomonas reinhardtii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.12.7.2 Fe 25.1 atoms per mol Chlamydomonas reinhardtii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.12.7.2 45000
-
 gel filtration Chlamydomonas reinhardtii
1.12.7.2 47500
-
 SDS-PAGE Chlamydomonas reinhardtii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.12.7.2 H2 + oxidized ferredoxin Chlamydomonas reinhardtii
-
 H+ + reduced ferredoxin
-
 r
1.12.7.2 H2 + oxidized ferredoxin Chlamydomonas reinhardtii 137 C(+)
-
 H+ + reduced ferredoxin
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.12.7.2 Chlamydomonas reinhardtii
-
-
-
1.12.7.2 Chlamydomonas reinhardtii 137 C(+)
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
1.12.7.2 rapid inactivation under aerobic conditions Chlamydomonas reinhardtii

Purification (Commentary)

EC Number Purification (Comment) Organism
1.12.7.2 partially, 40% pure Chlamydomonas reinhardtii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.12.7.2 1800
-
 hydrogen production, ferredoxin as electron donor Chlamydomonas reinhardtii

Storage Stability

EC Number Storage Stability Organism
1.12.7.2 -20°C, several months, strictly anaerobical storage, little loss of activity Chlamydomonas reinhardtii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.12.7.2 H2 + electron acceptor methyl viologen as electron acceptor Chlamydomonas reinhardtii H+ + reduced electron acceptor
-
 ?
1.12.7.2 H2 + electron acceptor sulfonatopropyl viologen, sodium metatungstate and sodium silicotungstate as electron acceptor Chlamydomonas reinhardtii H+ + reduced electron acceptor
-
 ?
1.12.7.2 H2 + electron acceptor methyl viologen as electron acceptor Chlamydomonas reinhardtii 137 C(+) H+ + reduced electron acceptor
-
 ?
1.12.7.2 H2 + electron acceptor sulfonatopropyl viologen, sodium metatungstate and sodium silicotungstate as electron acceptor Chlamydomonas reinhardtii 137 C(+) H+ + reduced electron acceptor
-
 ?
1.12.7.2 H2 + oxidized ferredoxin
-
 Chlamydomonas reinhardtii H+ + reduced ferredoxin
-
 r
1.12.7.2 H2 + oxidized ferredoxin
-
 Chlamydomonas reinhardtii 137 C(+) H+ + reduced ferredoxin
-
 r

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.12.7.2 25
-
 50% inactivation after 14 d Chlamydomonas reinhardtii
1.12.7.2 55
-
 50% inactivation after 1 h Chlamydomonas reinhardtii