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Literature summary extracted from

  • De Luca, G.; De Philip, P.; Rousset, M.; Belaich, J.P.; Dermoun, Z.
    The NADP-reducing hydrogenase of Desulfovibrio fructosovorans: evidence for a native complex with hydrogen-dependent methyl-viologen-reducing activity (1998), Biochem. Biophys. Res. Commun., 248, 591-596.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.12.1.3 HndA and HndB subunit Solidesulfovibrio fructosivorans

General Stability

EC Number General Stability Organism
1.12.1.3 50% loss of enzyme activity when extracts are exposed for 15 min to air Solidesulfovibrio fructosivorans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.12.1.3 0.09
-
 NADP+
-
 Solidesulfovibrio fructosivorans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.12.1.3 H2 + NADP+ Solidesulfovibrio fructosivorans biological function probably involves production of reduced pyridine nucleotides H+ + NADPH
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.12.1.3 Solidesulfovibrio fructosivorans
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.12.1.3 H2 + NADP+
-
 Solidesulfovibrio fructosivorans H+ + NADPH
-
 ?
1.12.1.3 H2 + NADP+ biological function probably involves production of reduced pyridine nucleotides Solidesulfovibrio fructosivorans H+ + NADPH
-
 ?
1.12.1.3 H2 + oxidized methyl viologen reduction requires all four subunits, a form lacking HndD shows no activity towards methyl viologen Solidesulfovibrio fructosivorans H+ + reduced methyl viologen
-
 ?

Subunits

EC Number Subunits Comment Organism
1.12.1.3 tetramer four different subunits with the following molecular weights: HndA 19000, HndB 14000, HndC 52000, HndD 64000, SDS-PAGE Solidesulfovibrio fructosivorans
1.12.1.3 tetramer four different subunits with the following molecular weights: HndA 18800, HndB 13800, HndC 52000, HndD 63400, SDS-PAGE Solidesulfovibrio fructosivorans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.12.1.3 8
-
-
 Solidesulfovibrio fructosivorans