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Literature summary extracted from

  • Porthun, A.; Bernhard, M.; Friedrich, B.
    Expression of a functional NAD-reducing [NiFe] hydrogenase from the gram-positive Rhodococcus opacus in the gram-negative Ralstonia eutropha (2002), Arch. Microbiol., 177, 159-166.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.12.1.2 heterologous expression in Ralstonia eutropha Rhodococcus opacus

Organism

EC Number Organism UniProt Comment Textmining
1.12.1.2 Rhodococcus opacus
-
-
-
1.12.1.2 Rhodococcus opacus MR11
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.12.1.2 1.7
-
 recombinant enzyme expressed in Ralstonia eutropha Rhodococcus opacus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.12.1.2 H2 + NAD+
-
 Rhodococcus opacus H+ + NADH
-
 r
1.12.1.2 H2 + NAD+
-
 Rhodococcus opacus MR11 H+ + NADH
-
 r
1.12.1.2 H2 + oxidized benzyl viologen
-
 Rhodococcus opacus H+ + reduced benzyl viologen
-
 r
1.12.1.2 H2 + oxidized benzyl viologen
-
 Rhodococcus opacus MR11 H+ + reduced benzyl viologen
-
 r
1.12.1.2 oxidized benzyl viologen + NADH
-
 Rhodococcus opacus reduced benzyl viologen + NAD+
-
 ?
1.12.1.2 oxidized benzyl viologen + NADH
-
 Rhodococcus opacus MR11 reduced benzyl viologen + NAD+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.12.1.2 More in low-salt buffer the tetrameric enzyme dissociates into 2 dimeric forms accompanied by the failure to reduce NAD+ Rhodococcus opacus