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Literature summary extracted from

  • Andrews, K.M.; Crofts, A.R.; Gennis, R.B.
    Large-scale purification and characterization of a highly active four-subunit cytochrome bc1 complex from Rhodobacter sphaeroides (1990), Biochemistry, 29, 2645-2651.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
7.1.1.8 dodecyl maltoside
-
 Paracoccus denitrificans
7.1.1.8 dodecyl maltoside
-
 Bos taurus
7.1.1.8 dodecyl maltoside
-
 Cereibacter sphaeroides
7.1.1.8 lecithin soybean Cereibacter sphaeroides

Cloned(Commentary)

EC Number Cloned (Comment) Organism
7.1.1.8 fbc operon cloned and sequenced Rhodobacter capsulatus

Inhibitors

EC Number Inhibitors Comment Organism Structure
7.1.1.8 Triton X-100
-
 Cereibacter sphaeroides

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
7.1.1.8 chromatophore inner surface of the chromatophore membrane Cereibacter sphaeroides
-
-
7.1.1.8 mitochondrion
-
 Bos taurus 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
7.1.1.8 Iron-sulfur cluster
-
 Bos taurus
7.1.1.8 Iron-sulfur cluster
-
 Cereibacter sphaeroides

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
7.1.1.8 14000
-
 1 * 40000 + 1 * 34000 + 1 * 24000 + 1 * 14000, SDS-PAGE Cereibacter sphaeroides
7.1.1.8 24000
-
 1 * 40000 + 1 * 34000 + 1 * 24000 + 1 * 14000, SDS-PAGE Cereibacter sphaeroides
7.1.1.8 34000
-
 1 * 40000 + 1 * 34000 + 1 * 24000 + 1 * 14000, SDS-PAGE Cereibacter sphaeroides
7.1.1.8 40000
-
 1 * 40000 + 1 * 34000 + 1 * 24000 + 1 * 14000, SDS-PAGE Cereibacter sphaeroides

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
7.1.1.8 ubiquinone-10 + ferricytochrome c Paracoccus denitrificans
-
 ubiquinol-10 + ferrocytochrome c + 2 H+
-
 ?
7.1.1.8 ubiquinone-10 + ferricytochrome c Bos taurus
-
 ubiquinol-10 + ferrocytochrome c + 2 H+
-
 ?
7.1.1.8 ubiquinone-10 + ferricytochrome c Cereibacter sphaeroides
-
 ubiquinol-10 + ferrocytochrome c + 2 H+
-
 ?
7.1.1.8 ubiquinone-10 + ferricytochrome c Rhodobacter capsulatus
-
 ubiquinol-10 + ferrocytochrome c + 2 H+
-
 ?
7.1.1.8 ubiquinone-10 + ferricytochrome c Bacillus sp. (in: Bacteria)
-
 ubiquinol-10 + ferrocytochrome c + 2 H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
7.1.1.8 Bos taurus
-
-
-
7.1.1.8 Cereibacter sphaeroides
-
-
-
7.1.1.8 Paracoccus denitrificans
-
-
-
7.1.1.8 Rhodobacter capsulatus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
7.1.1.8 phospholipoprotein
-
 Paracoccus denitrificans
7.1.1.8 phospholipoprotein
-
 Bos taurus
7.1.1.8 phospholipoprotein
-
 Cereibacter sphaeroides

Purification (Commentary)

EC Number Purification (Comment) Organism
7.1.1.8
-
 Cereibacter sphaeroides

Source Tissue

EC Number Source Tissue Comment Organism Textmining
7.1.1.8 heart
-
 Bos taurus
-

Storage Stability

EC Number Storage Stability Organism
7.1.1.8 -70°C, 50 mM Tris-HCl buffer, pH 8.0, 200 mM NaCl, 0.1 mg/ml dodecylmaltoside, 50% glycerol Cereibacter sphaeroides

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.1.1.8 quinol + 2 horse heart ferricytochrome c1
-
 Cereibacter sphaeroides quinone + 2 horse heart ferrocytochrome c1 + H+ [side 2]
-
 ?
7.1.1.8 ubiquinol + 2 ferricytochrome c
-
 Paracoccus denitrificans ubiquinone + 2 ferrocytochrome c + 2 H+
-
 ?
7.1.1.8 ubiquinol + 2 ferricytochrome c
-
 Bos taurus ubiquinone + 2 ferrocytochrome c + 2 H+
-
 ?
7.1.1.8 ubiquinol + 2 ferricytochrome c
-
 Cereibacter sphaeroides ubiquinone + 2 ferrocytochrome c + 2 H+
-
 ?
7.1.1.8 ubiquinol + 2 ferricytochrome c
-
 Rhodobacter capsulatus ubiquinone + 2 ferrocytochrome c + 2 H+
-
 ?
7.1.1.8 ubiquinol + 2 ferricytochrome c
-
 Bacillus sp. (in: Bacteria) ubiquinone + 2 ferrocytochrome c + 2 H+
-
 ?
7.1.1.8 ubiquinol + horse heart cytochrome c
-
 Cereibacter sphaeroides ?
-
 ?
7.1.1.8 ubiquinone-10 + ferricytochrome c
-
 Paracoccus denitrificans ubiquinol-10 + ferrocytochrome c + 2 H+
-
 ?
7.1.1.8 ubiquinone-10 + ferricytochrome c
-
 Bos taurus ubiquinol-10 + ferrocytochrome c + 2 H+
-
 ?
7.1.1.8 ubiquinone-10 + ferricytochrome c
-
 Cereibacter sphaeroides ubiquinol-10 + ferrocytochrome c + 2 H+
-
 ?
7.1.1.8 ubiquinone-10 + ferricytochrome c
-
 Rhodobacter capsulatus ubiquinol-10 + ferrocytochrome c + 2 H+
-
 ?
7.1.1.8 ubiquinone-10 + ferricytochrome c
-
 Bacillus sp. (in: Bacteria) ubiquinol-10 + ferrocytochrome c + 2 H+
-
 ?

Subunits

EC Number Subunits Comment Organism
7.1.1.8 tetramer
-
 Rhodobacter capsulatus
7.1.1.8 tetramer 1 * 40000 + 1 * 34000 + 1 * 24000 + 1 * 14000, SDS-PAGE Cereibacter sphaeroides

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
7.1.1.8 4.17 5 2 horse heart ferricytochrome c1
-
 Cereibacter sphaeroides
7.1.1.8 25 35 cytochrome c
-
 Cereibacter sphaeroides

Cofactor

EC Number Cofactor Comment Organism Structure
7.1.1.8 heme b prosthetic group Cereibacter sphaeroides
7.1.1.8 heme c prosthetic group Cereibacter sphaeroides
7.1.1.8 Phospholipid
-
 Bos taurus
7.1.1.8 Phospholipid prosthetic group Cereibacter sphaeroides
7.1.1.8 semiquinone antimycin-sensitive prosthetic group Cereibacter sphaeroides
7.1.1.8 ubiquinone
-
 Bos taurus
7.1.1.8 ubiquinone prosthetic group Cereibacter sphaeroides