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Literature summary extracted from

  • Guergova-Kuras, M.; Salcedo-Hernandez, R.; Bechmann, G.; Kuras, R.; Gennis, R.B.; Crofts, A.R.
    Expression and one-step purification of a fully active polyhistidine-tagged cytochrome bc1 complex from Rhodobacter sphaeroides (1999), Protein Expr. Purif., 15, 370-380.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
7.1.1.8 fbcB and fbcC genes cloned using PCR amplification Cereibacter sphaeroides

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
7.1.1.8 chromatophore
-
 Cereibacter sphaeroides
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
7.1.1.8 14000
-
 1 * 40100 + 1 * 32600 + 1 * 19000 + 1 * 14000, SDS-PAGE Cereibacter sphaeroides
7.1.1.8 19000
-
 FeS-protein, SDS-PAGE Cereibacter sphaeroides
7.1.1.8 26000
-
 gel filtration Cereibacter sphaeroides
7.1.1.8 32600
-
 1 * 40100 + 1 * 32600 + 1 * 19000 + 1 * 14000, SDS-PAGE Cereibacter sphaeroides
7.1.1.8 34100
-
 His-tagged cyt c1, SDS-PAGE Cereibacter sphaeroides
7.1.1.8 40100
-
 1 * 40100 + 1 * 32600 + 1 * 19000 + 1 * 14000, SDS-PAGE Cereibacter sphaeroides
7.1.1.8 40800
-
 His-tagged cyt b, SDS-PAGE Cereibacter sphaeroides

Organism

EC Number Organism UniProt Comment Textmining
7.1.1.8 Cereibacter sphaeroides
-
 and strains BH6, CH6
-
7.1.1.8 Cereibacter sphaeroides BC17
-
 and strains BH6, CH6
-

Purification (Commentary)

EC Number Purification (Comment) Organism
7.1.1.8
-
 Cereibacter sphaeroides

Storage Stability

EC Number Storage Stability Organism
7.1.1.8 -80°C, lyophilized in 50% v/v glycerol Cereibacter sphaeroides

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.1.1.8 ubiquinol + 2 ferricytochrome c
-
 Cereibacter sphaeroides ubiquinone + 2 ferrocytochrome c + 2 H+
-
 ?
7.1.1.8 ubiquinol + 2 ferricytochrome c
-
 Cereibacter sphaeroides BC17 ubiquinone + 2 ferrocytochrome c + 2 H+
-
 ?

Subunits

EC Number Subunits Comment Organism
7.1.1.8 dimer
-
 Cereibacter sphaeroides
7.1.1.8 tetramer 1 * 40100 + 1 * 32600 + 1 * 19000 + 1 * 14000, SDS-PAGE Cereibacter sphaeroides

Synonyms

EC Number Synonyms Comment Organism
7.1.1.8 bc1 complex
-
 Cereibacter sphaeroides