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Literature summary extracted from
- Bifunctional abietadiene synthase: Free diffusive transfer of the (+)-copalyl diphosphate intermediate between two distinct active sites (2001), J. Am. Chem. Soc., 123, 8974-8978.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 5.5.1.12 | expression in Escherichia coli | Abies grandis |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 4.2.3.18 | D404A | unreactive with geranylgeranyl diphosphate | Abies grandis |
| 4.2.3.18 | D621A | unreactive with (+)-copalyl diphosphate | Abies grandis |
| 5.5.1.12 | D404A | unreactive with geranylgeranyl diphosphate | Abies grandis |
| 5.5.1.12 | D621A | unreactive with (+)-copalyl diphosphate | Abies grandis |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.3.18 | 14,15-dihydro-15-azageranylgeranyl diphosphate | - |
Abies grandis |  |
| 4.2.3.18 | geranylgeranyl diphosphate | substrate inhibition | Abies grandis | |
| 5.5.1.12 | 14,15-dihydro-15-azageranylgeranyl diphosphate | - |
Abies grandis | |
| 5.5.1.12 | geranylgeranyl diphosphate | - |
Abies grandis | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.2.3.18 | 0.00035 | - |
copalyl diphosphate | - |
Abies grandis | |
| 4.2.3.18 | 0.0004 | - |
copalyl diphosphate | D404A | Abies grandis | |
| 4.2.3.18 | 132 | - |
copalyl diphosphate | - |
Abies grandis | |
| 4.2.3.18 | 138 | - |
copalyl diphosphate | mutant D404A | Abies grandis | |
| 5.5.1.12 | 0.003 | - |
geranylgeranyl diphosphate | - |
Abies grandis | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.2.3.18 | 90000 | - |
gel filtration | Abies grandis |
| 5.5.1.12 | 90000 | - |
gel filtration | Abies grandis |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.3.18 | (+)-copalyl-diphosphate | Abies grandis | - |
(-)-abietadiene + diphosphate | - |
? | |
| 5.5.1.12 | geranylgeranyl diphosphate | Abies grandis | - |
(+)-copalyl diphosphate | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.2.3.18 | Abies grandis | - |
- |
- |
| 5.5.1.12 | Abies grandis | - |
- |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.3.18 | (+)-copalyl-diphosphate | - |
Abies grandis | (-)-abietadiene + diphosphate | - |
? | |
| 5.5.1.12 | geranylgeranyl diphosphate | - |
Abies grandis | (+)-copalyl diphosphate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 4.2.3.18 | monomer | - |
Abies grandis |
| 5.5.1.12 | monomer | - |
Abies grandis |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.5.1.12 | 2.2 | - |
geranylgeranyl diphosphate | - |
Abies grandis | |
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