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Literature summary extracted from
- 25-Hydroxycholecalciferol-1-hydroxylase. Subcellular location and properties (1972), J. Biol. Chem., 247, 7528-7532.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.15.18 | malate | supports 1alpha-hydroxylation as electron donor | Gallus gallus |  |
| 1.14.15.18 | succinate | supports 1alpha-hydroxylation as electron donor | Gallus gallus | |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.15.18 | 1,25-Dihydroxycholecalciferol | calcitriol | Gallus gallus | |
| 1.14.15.18 | 25-hydroxydihydrotachysterol3 | - |
Gallus gallus | |
| 1.14.15.18 | antimycin A | - |
Gallus gallus | |
| 1.14.15.18 | CO | 50% O2/50% CO2 incubation atmosphere | Gallus gallus | |
| 1.14.15.18 | cyanide | - |
Gallus gallus | |
| 1.14.15.18 | dinitrophenol | - |
Gallus gallus | |
| 1.14.15.18 | diphenyl-p-phenylenediamine | - |
Gallus gallus | |
| 1.14.15.18 | Metyrapone | - |
Gallus gallus | |
| 1.14.15.18 | oligomycin | - |
Gallus gallus | |
| 1.14.15.18 | p-chloromercuribenzoate | - |
Gallus gallus | |
| 1.14.15.18 | phosphate | - |
Gallus gallus | |
| 1.14.15.18 | sucrose | hypertonic | Gallus gallus | |
| 1.14.15.18 | Tris-chloride buffer | - |
Gallus gallus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.15.18 | 0.0022 | - |
25-hydroxycholecalciferol | - |
Gallus gallus | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.14.15.18 | mitochondrion | - |
Gallus gallus | 5739 | - |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.15.18 | Mg2+ | required | Gallus gallus | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.15.18 | 25-hydroxycholecalciferol + NADPH + O2 | Gallus gallus | central role in calcium regulation | calcitriol + NADP+ + H2O | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.15.18 | Gallus gallus | - |
- |
- |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.14.15.18 | kidney | - |
Gallus gallus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.14.15.18 | 0.00125 | - |
- |
Gallus gallus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.15.18 | 25-hydroxycholecalciferol + NADPH + O2 | central role in calcium regulation | Gallus gallus | calcitriol + NADP+ + H2O | - |
? | |
| 1.14.15.18 | calcidiol + NADPH + O2 | highly specific for calcidiol, calcidiol is identical with 25-hydroxycholecalciferol | Gallus gallus | calcitriol + NADP+ + H2O | calcitriol is identical with 1,25-dihydroxycholecalciferol | ? | |
| 1.14.15.18 | additional information | not: cholecalciferol, dihydrotachysterol | Gallus gallus | ? | - |
? | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.15.18 | 7.4 | - |
- |
Gallus gallus |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.15.18 | 7.1 | 7.7 | about 50% of activity maximum at pH 7.1 and pH 7.7 | Gallus gallus |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.15.18 | NADPH | - |
Gallus gallus | |
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