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Literature summary extracted from

  • Lobaugh, B.; Almond, J.R.; Drezner, M.K.
    Measurement of 25-hydroxyvitamin D 1-alpha-hydroxylase activity in mammalian kidney (1986), Methods Enzymol., 123, 159-167.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.15.18 malate supports 1alpha-hydroxylation as electron donor Mammalia
1.14.15.18 succinate supports 1alpha-hydroxylation as electron donor Mammalia

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.15.18 antimycin A
-
 Mammalia
1.14.15.18 CN-
-
 Mammalia
1.14.15.18 CO
-
 Mammalia
1.14.15.18 cyanide
-
 Mammalia
1.14.15.18 dinitrophenol
-
 Mammalia
1.14.15.18 Glutethimide
-
 Mammalia
1.14.15.18 Metyrapone
-
 Mammalia
1.14.15.18 Natural inhibitors vitamin D binding protein Mammalia
1.14.15.18 rotenone
-
 Mammalia

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.15.18 additional information almost any other Krebs cycle substrate supports 1-alpha-hydroxylation Mammalia

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.15.18 additional information
-
 ferredoxin, ferredoxin reductase and cytochrome P-450 Mammalia

Organism

EC Number Organism UniProt Comment Textmining
1.14.15.18 Mammalia
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.14.15.18 calcidiol + 2 reduced adrenodoxin + 2 H+ + O2 = calcitriol + 2 oxidized adrenodoxin + H2O 25-hydroxyvitamin D3 1alpha-hydroxylase is a cytochrome P-450-dependent mixed-function oxidase Mammalia
a
1.14.15.18 calcidiol + 2 reduced adrenodoxin + 2 H+ + O2 = calcitriol + 2 oxidized adrenodoxin + H2O ferredoxin, ferredoxin reductase and cytochrome P-450 Mammalia
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.15.18 kidney
-
 Mammalia
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.15.18 calcidiol + NADPH + O2 highly specific for calcidiol, calcidiol is identical with 25-hydroxycholecalciferol Mammalia calcitriol + NADP+ + H2O calcitriol is identical with 1,25-dihydroxycholecalciferol ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.15.18 NADPH
-
 Mammalia