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Literature summary extracted from

  • Sakaki, T.; Sawada, N.; Takeyama, K.i.; Kato, S.; Inouye, K.
    Enzymic properties of mouse 25-hydroxyvitamin D3 1alpha-hydroxylase expressed in Escherichia coli (1999), Eur. J. Biochem., 259, 731-738.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.15.18 expression in Escherichia coli Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.15.18 CO
-
 Mus musculus
1.14.15.18 ketoconazole
-
 Mus musculus
1.14.15.18 Metyrapone
-
 Mus musculus
1.14.15.18 vitamin D3
-
 Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.15.18 0.0013
-
 24,25-dihydroxycholecalciferol
-
 Mus musculus
1.14.15.18 0.0027
-
 25-hydroxycholecalciferol
-
 Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.15.18 25-hydroxycholecalciferol + NADPH + O2 Mus musculus recombinant in E. coli calcitriol + NADP+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.15.18 Mus musculus
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.14.15.18 calcidiol + 2 reduced adrenodoxin + 2 H+ + O2 = calcitriol + 2 oxidized adrenodoxin + H2O cytochrome P-450 enzyme Mus musculus
a
1.14.15.18 calcidiol + 2 reduced adrenodoxin + 2 H+ + O2 = calcitriol + 2 oxidized adrenodoxin + H2O reconstituted with cytochrome P-450, adrenal ferredoxin, i.e. adrenodoxin, and adrenodoxin reductase Mus musculus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.15.18 kidney
-
 Mus musculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.15.18 0.0000002
-
 substrate 23,25-dihydroxycholecalciferol Mus musculus
1.14.15.18 0.0000011
-
 substrate 24-oxo-25-hydroxycholecalciferol Mus musculus
1.14.15.18 0.0000015
-
-
 Mus musculus
1.14.15.18 0.0000043
-
 substrate 24,25-dihydroxycholecalciferol Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.15.18 (24R),25-dihydroxycholecalciferol + NADPH + O2 recombinant enzyme Mus musculus 1alpha,24,25-trihydroxycholecalciferol + NADP+ + H2O
-
 ?
1.14.15.18 (24R),25-dihydroxycholecalciferol + NADPH + O2 higher activity than with 25-hydroxycholecalciferol Mus musculus 1alpha,24,25-trihydroxycholecalciferol + NADP+ + H2O
-
 ?
1.14.15.18 23,25-dihydroxycholecalciferol + NADPH + O2 low activity Mus musculus ?
-
 ?
1.14.15.18 23,25-dihydroxycholecalciferol + NADPH + O2 recombinant enzyme Mus musculus ?
-
 ?
1.14.15.18 24,25-dihydroxycholecalciferol + reduced adrenodoxin + O2
-
 Mus musculus 1alpha,24,25-trihydroxycholecalciferol + oxidized adrenodoxin + H2O
-
 r
1.14.15.18 24-oxo-23,25-dihydroxycholecalciferol + NADPH + O2 low activity Mus musculus ?
-
 ?
1.14.15.18 24-oxo-23,25-dihydroxycholecalciferol + NADPH + O2 recombinant enzyme Mus musculus ?
-
 ?
1.14.15.18 24-oxo-25-hydroxycholecalciferol + NADPH + O2 recombinant enzyme Mus musculus ?
-
 ?
1.14.15.18 25-hydroxycholecalciferol + NADPH + O2 recombinant in E. coli Mus musculus calcitriol + NADP+ + H2O
-
 ?
1.14.15.18 calcidiol + NADPH + O2 recombinant enzyme Mus musculus calcitriol + NADP+ + H2O calcitriol is identical with 1,25-dihydroxycholecalciferol ?
1.14.15.18 calcidiol + NADPH + O2 highly specific for calcidiol, calcidiol is identical with 25-hydroxycholecalciferol Mus musculus calcitriol + NADP+ + H2O calcitriol is identical with 1,25-dihydroxycholecalciferol ?
1.14.15.18 additional information the 25-hydroxy group is essential for 1alpha-hydroxylating activity, the 24-hydroxy group enhances the activity, the 23-hydroxy group strongly reduces activity Mus musculus ?
-
 ?
1.14.15.18 additional information no activity with 24,25,26,27-tetranor-23-hydroxyvitamin D3 and vitamin D3 Mus musculus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.15.18 CYB27B1
-
 Mus musculus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.15.18 37
-
 assay at Mus musculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.15.18 7.4
-
 assay at Mus musculus

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.15.18 NADPH
-
 Mus musculus