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Literature summary extracted from

  • Tso, M.Y.W.
    Some properties of the nitrogenase proteins from Clostridium pasteurianum. Molecular weight, subunit structure, isoelectric point and EPR spectra (1974), Arch. Microbiol., 99, 71-80.
    View publication on PubMed

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.18.6.1 Mg2+ Mg2+ required for MgATP complex Clostridium pasteurianum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.18.6.1 27500
-
2 * 27500, SDS-PAGE Clostridium pasteurianum
1.18.6.1 27500
-
component I Fe protein 2 * 27500 + component II MoFe protein 1 * 60000 + 1 * 51000, SDS-PAGE Clostridium pasteurianum
1.18.6.1 51000
-
component I Fe protein 2 * 27500 + component II MoFe protein 1 * 60000 + 1 * 51000, SDS-PAGE Clostridium pasteurianum
1.18.6.1 56000
-
Fe protein, gel filtration Clostridium pasteurianum
1.18.6.1 60000
-
component I Fe protein 2 * 27500 + component II MoFe protein 1 * 60000 + 1 * 51000, SDS-PAGE Clostridium pasteurianum
1.18.6.1 210000
-
MoFe protein, gel filtration Clostridium pasteurianum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.18.6.1 8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O Clostridium pasteurianum
-
8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.18.6.1 Clostridium pasteurianum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.18.6.1 all components Clostridium pasteurianum

Reaction

EC Number Reaction Comment Organism Reaction ID
1.18.6.1 4 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 4 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate enzyme is composed of 2 metalloproteins: component I MoFe protein and component II Fe protein Clostridium pasteurianum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.18.6.1 8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O
-
Clostridium pasteurianum 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate
-
?

Subunits

EC Number Subunits Comment Organism
1.18.6.1 dimer 2 * 27500, SDS-PAGE Clostridium pasteurianum
1.18.6.1 tetramer component I Fe protein 2 * 27500 + component II MoFe protein 1 * 60000 + 1 * 51000, SDS-PAGE Clostridium pasteurianum