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Literature summary extracted from

  • White, K.A.; Marletta, M.A.
    Nitric oxide synthase is a cytochrome P-450 type hemoprotein (1992), Biochemistry, 31, 6627-6631.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.13.39 CO
-
 Mus musculus
1.14.13.39 CO partially purified rat cerebellum enzyme Rattus norvegicus
1.14.13.39 CO/O2 80%:20%, mixture Mus musculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.13.39 cytosol
-
 Mus musculus 5829
-
1.14.13.39 cytosol
-
 Rattus norvegicus 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.13.39 Iron mouse macrophage enzyme: cytochrome P-450 type hemoprotein Mus musculus
1.14.13.39 Iron protoporphyrin IX heme Mus musculus
1.14.13.39 Iron protoporphyrin IX heme Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.13.39 2 L-arginine + 3 NADPH + 4 O2 + 3 H+ Mus musculus soluble cytochrome P-450 enzyme in eukaryotes 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O
-
 ?
1.14.13.39 2 L-arginine + 3 NADPH + 4 O2 + 3 H+ Rattus norvegicus soluble cytochrome P-450 enzyme in eukaryotes 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.39 Mus musculus
-
-
-
1.14.13.39 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.39 2',5'-ADP-agarose affinity chromatography Mus musculus
1.14.13.39 2',5'-ADP-agarose affinity chromatography Rattus norvegicus
1.14.13.39 partial Rattus norvegicus

Reaction

EC Number Reaction Comment Organism Reaction ID
1.14.13.39 2 L-arginine + 3 NADPH + 3 H+ + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O possible mechanism Mus musculus
a
1.14.13.39 2 L-arginine + 3 NADPH + 3 H+ + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O possible mechanism Rattus norvegicus
a
1.14.13.39 2 L-arginine + 3 NADPH + 3 H+ + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O cytochrome P-450 enzyme Mus musculus
a
1.14.13.39 2 L-arginine + 3 NADPH + 3 H+ + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O cytochrome P-450 enzyme Rattus norvegicus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.13.39 brain
-
 Mus musculus
-
1.14.13.39 brain
-
 Rattus norvegicus
-
1.14.13.39 cerebellum
-
 Rattus norvegicus
-
1.14.13.39 macrophage
-
 Mus musculus
-
1.14.13.39 macrophage
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.13.39 additional information
-
-
 Mus musculus
1.14.13.39 additional information
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.39 2 L-arginine + 3 NADPH + 4 O2 + 3 H+ specific for NADPH, 5-electron oxidation of L-arginine Mus musculus 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O
-
 ?
1.14.13.39 2 L-arginine + 3 NADPH + 4 O2 + 3 H+ specific for NADPH, 5-electron oxidation of L-arginine Rattus norvegicus 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O
-
 ?
1.14.13.39 2 L-arginine + 3 NADPH + 4 O2 + 3 H+ soluble cytochrome P-450 enzyme in eukaryotes Mus musculus 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O
-
 ?
1.14.13.39 2 L-arginine + 3 NADPH + 4 O2 + 3 H+ soluble cytochrome P-450 enzyme in eukaryotes Rattus norvegicus 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.13.39 More significant amino acid sequence homology to NADPH-cytochrome P-450-type hemoprotein Mus musculus
1.14.13.39 More significant amino acid sequence homology to NADPH-cytochrome P-450-type hemoprotein Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.13.39 37
-
 assay at Mus musculus
1.14.13.39 37
-
 assay at Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.39 5,6,7,8-tetrahydro-L-biopterin
-
 Mus musculus
1.14.13.39 5,6,7,8-tetrahydro-L-biopterin
-
 Rattus norvegicus
1.14.13.39 NADPH
-
 Mus musculus
1.14.13.39 NADPH
-
 Rattus norvegicus