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Literature summary extracted from

  • Yum, D.Y.; Lee, B.Y.; Pan, J.G.
    Identification of the yqhE and yafB genes encoding two 2,5-diketo-D-gluconate reductases in Escherichia coli (1999), Appl. Environ. Microbiol., 65, 3341-3346.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
1.1.1.274 synthesis enzyme may be useful for the synthesis of the ascorbate precursor 2-keto-L-gulonate Escherichia coli

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.274 overexpression of isoenzymes A and B Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.274 29000
-
 1 * 29000, isoenzymes A and B, SDS-PAGE Escherichia coli
1.1.1.274 30000
-
 isoenzymes A and B, gel filtration Escherichia coli
1.1.1.346 29000
-
 1 * 29000, SDS-PAGE Escherichia coli
1.1.1.346 30000
-
 gel filtration Escherichia coli
1.1.1.346 31003
-
 1 * 31003, calculated from amino acid sequence Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.274 Escherichia coli P30863
-
-
1.1.1.346 Escherichia coli
-
-
-
1.1.1.346 Escherichia coli Q46857
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.274 recombinant isoenzymes A and B, affinity chitin-binding tag Escherichia coli
1.1.1.346 Ni-NTA column chromatography Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.274 2,5-didehydro-D-gluconate + NADPH both isoenzymes A and B are specific for NADPH Escherichia coli 2-keto-L-gulonate + NADP+
-
 ?
1.1.1.346 2,5-didehydro-D-gluconate + NADPH + H+ NADPH is the preferred electron donor Escherichia coli 2-dehydro-L-gulonate + NADP+
-
 ?
1.1.1.346 additional information 2-keto-D-gluconate, 2-keto-L-gulonate, 5-keto-D-gluconate, D-fructose, and L-sorbose are inactive when assayed at pH 6.0 in the presence of NADH or NADPH Escherichia coli ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.274 monomer 1 * 29000, isoenzymes A and B, SDS-PAGE Escherichia coli
1.1.1.346 monomer 1 * 29000, SDS-PAGE Escherichia coli
1.1.1.346 monomer 1 * 31003, calculated from amino acid sequence Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
1.1.1.346 2,5-diketo-D-gluconate reductase
-
 Escherichia coli
1.1.1.346 2,5-diketo-D-gluconate-reductase
-
 Escherichia coli
1.1.1.346 25DKGR-A
-
 Escherichia coli
1.1.1.346 dkgA
-
 Escherichia coli
1.1.1.346 dkgB
-
 Escherichia coli
1.1.1.346 YqhE
-
 Escherichia coli
1.1.1.346 YqhE reductase
-
 Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.274 7
-
 isoenzyme B Escherichia coli
1.1.1.274 7.5
-
 isoenzyme A Escherichia coli
1.1.1.346 7.5
-
-
 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.274 NADP+
-
 Escherichia coli
1.1.1.274 NADPH
-
 Escherichia coli