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Literature summary extracted from

  • Yorita, K.; Aki, K.; Ohkuma-Soyejima, T.; Kokubo, T.; Misaki, H.; Massey, V.
    Conversion of L-lactate oxidase to a long chain alpha-hydroxyacid oxidase by site-directed mutagenesis of alanine 95 to glycine (1996), J. Biol. Chem., 271, 28300-28305.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.3.15 expressed in Escherichia coli DH1 Aerococcus viridans

Protein Variants

EC Number Protein Variants Comment Organism
1.1.3.15 A95G much enhanced oxidase activity with longer chain L-alpha-hydroxyacids such as alpha-hydroxy-n-butyric acid, alpha-hydroxy-n-valeric acid and L-mandelic acid Aerococcus viridans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.15 0.3
-
 L-Mandelate
-
 Aerococcus viridans
1.1.3.15 0.87
-
 (S)-lactate
-
 Aerococcus viridans
1.1.3.15 5
-
 DL-glycerate
-
 Aerococcus viridans
1.1.3.15 5.5
-
 DL-alpha-hydroxy-n-valerate
-
 Aerococcus viridans
1.1.3.15 6.75
-
 (S)-lactate A95G mutant Aerococcus viridans
1.1.3.15 10
-
 DL-alpha-hydroxy-n-valerate A95G mutant Aerococcus viridans
1.1.3.15 18
-
 DL-alpha-hydroxy-n-butyrate
-
 Aerococcus viridans
1.1.3.15 20
-
 L-Mandelate A95G mutant Aerococcus viridans
1.1.3.15 27
-
 DL-alpha-hydroxy-n-butyrate A95G mutant Aerococcus viridans
1.1.3.15 53
-
 DL-glycerate A95G mutant Aerococcus viridans
1.1.3.15 125
-
 L-alpha-hydroxy-isovalerate A95G mutant Aerococcus viridans
1.1.3.15 140
-
 L-alpha-hydroxy-beta-methylvalerate A95G mutant Aerococcus viridans

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.15 Aerococcus viridans
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.15 2-hydroxy-3-methylvalerate + O2 A95G-mutant Aerococcus viridans ?
-
 ?
1.1.3.15 2-hydroxybutyrate + O2 wild-type and A95G-mutant Aerococcus viridans ?
-
 ?
1.1.3.15 2-hydroxyisovalerate + O2 A95G-mutant Aerococcus viridans ?
-
 ?
1.1.3.15 2-hydroxyvalerate + O2 wild-type and A95G-mutant Aerococcus viridans ?
-
 ?
1.1.3.15 DL-glycerate + O2
-
 Aerococcus viridans ?
-
 r
1.1.3.15 L-2-hydroxy-beta-methylvalerate + 2,6-dichlorophenolindophenol
-
 Aerococcus viridans 3-methyl-2-oxopentanoate + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.3.15 L-alpha-hydroxy-isovalerate + O2
-
 Aerococcus viridans 3-methyl-2-oxobutanoate + H2O2
-
 r
1.1.3.15 L-mandelate + O2 A95G-mutant is also reactive Aerococcus viridans ?
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.3.15 FMN
-
 Aerococcus viridans