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Literature summary extracted from

  • Mann, J.D.; Mudd, S.H.
    Alkaloids and plant metabolism (1963), J. Biol. Chem., 238, 381-385.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.27 Homotyramine
-
 Hordeum vulgare
2.1.1.27 additional information EDTA is not inhibitory Hordeum vulgare
2.1.1.27 N-Methyltyramine
-
 Hordeum vulgare
2.1.1.27 p-chloromercuribenzoate strong Hordeum vulgare

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.27 0.02
-
 tyramine
-
 Hordeum vulgare
2.1.1.27 0.03
-
 S-adenosyl-L-methionine
-
 Hordeum vulgare

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.27 additional information Hordeum vulgare involved in hordenine biosynthesis ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.27 Hordeum vulgare
-
 L. var. Betzes, barley
-

Oxidation Stability

EC Number Oxidation Stability Organism
2.1.1.27 sulfhydryl compound necessary for stabilization Hordeum vulgare

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.27 partial Hordeum vulgare

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.27 root of seedling Hordeum vulgare
-

Storage Stability

EC Number Storage Stability Organism
2.1.1.27 -18°C, addition of sucrose to a final concentration of 0.2 M, several weeks Hordeum vulgare
2.1.1.27 4°C, pH 7.0 and 5 mM 2-mercaptoethanol, several days Hordeum vulgare

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.27 4-chlorophenethylamine + S-adenosyl-L-methionine 29% of activity with tyramine Hordeum vulgare ?
-
 ?
2.1.1.27 beta-phenethanolamine + S-adenosyl-L-methionine 23% of activity with tyramine Hordeum vulgare ?
-
 ?
2.1.1.27 additional information involved in hordenine biosynthesis Hordeum vulgare ?
-
 ?
2.1.1.27 S-adenosyl-L-methionine + alpha-methyltyramine i.e. paredrine, 53% of activity with tyramine Hordeum vulgare ?
-
 ?
2.1.1.27 S-adenosyl-L-methionine + tyramine conversion rate is higher with (-)-L-diastereoisomer of methionine Hordeum vulgare N-methyltyramine + S-adenosyl-L-homocysteine
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.27 26
-
 assay at Hordeum vulgare

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.1.1.27 37
-
 heat-labile: 70% loss of activity after 10 min Hordeum vulgare

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.27 8.4
-
-
 Hordeum vulgare

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.27 additional information no cofactor requirement Hordeum vulgare