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Literature summary extracted from

  • Speedie, M.K.; Hornemann, U.; Floss, H.G.
    Isolation and characterization of tryptophan transaminase and indolepyruvate C-methyltransferase. Enzymes involved in indolmycin biosynthesis in Streptomyces griseus (1975), J. Biol. Chem., 250, 7819-7825.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.1.1.47 freezing inactivates Streptomyces griseus
2.1.1.47 heating inactivates Streptomyces griseus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.47 1,10-phenanthroline
-
Streptomyces griseus
2.1.1.47 2,2'-bipyridyl
-
Streptomyces griseus
2.1.1.47 iodoacetamide weak Streptomyces griseus
2.1.1.47 iodoacetic acid weak Streptomyces griseus
2.1.1.47 additional information no inhibition by EDTA; not inhibitory: indolmycin, L-tryptophan, diethyldithiocarbamate, L-tryptophanylglycine, L-tryptophanamide Streptomyces griseus
2.1.1.47 N-ethylmaleimide
-
Streptomyces griseus
2.1.1.47 p-chloromercuribenzoate strong, reversible by cysteine Streptomyces griseus
2.6.1.27 3-Methyltryptophan can also act as substrate Streptomyces griseus
2.6.1.27 p-chloromercuribenzoate no inhibition Streptomyces griseus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.47 0.0048
-
Indolepyruvate
-
Streptomyces griseus
2.1.1.47 0.013
-
S-adenosyl-L-methionine
-
Streptomyces griseus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.1.1.47 additional information no requirement for Mg2+, Mn2+, Ca2+ Streptomyces griseus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.47 55000
-
gel filtration Streptomyces griseus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.47 S-adenosyl-L-methionine + indolepyruvate Streptomyces griseus reaction in indolmycin biosynthesis ?
-
?
2.6.1.27 L-tryptophan + 2-oxoglutarate Streptomyces griseus initial step of biosynthetic pathway of the antibiotic indolmycin L-glutamate + 3-indole-2-oxopropanoate
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.47 Streptomyces griseus
-
-
-
2.6.1.27 Streptomyces griseus
-
strain ATCC 12648
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.47
-
Streptomyces griseus
2.6.1.27 partial, 3fold Streptomyces griseus

Storage Stability

EC Number Storage Stability Organism
2.1.1.47 2°C, up to 1 month Streptomyces griseus
2.6.1.27 -20°C, best stored by freezing the crude cell-free extract with 10% glycerol or by freezing the 45-60% ammonium sulfate precipitate in a phosphate buffer solution Streptomyces griseus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.47 additional information no substrate: L-tryptophan Streptomyces griseus ?
-
?
2.1.1.47 additional information no substrates are phenylpyruvate, p-hydroxyphenylpyruvate Streptomyces griseus ?
-
?
2.1.1.47 S-adenosyl-L-methionine + indolepyruvate
-
Streptomyces griseus S-adenosyl-L-homocysteine + (S)-beta-methylindolepyruvate
-
?
2.1.1.47 S-adenosyl-L-methionine + indolepyruvate reaction in indolmycin biosynthesis Streptomyces griseus ?
-
?
2.6.1.27 3-methyltryptophan + 2-oxoglutarate can also inhibit the enzyme, stereospecific for positions 2 and 3 Streptomyces griseus 3-(3-methylindole)-2-oxopropanoate + L-glutamate
-
?
2.6.1.27 L-phenylalanine + 2-oxoglutarate
-
Streptomyces griseus L-glutamate + phenylpyruvate
-
?
2.6.1.27 L-tryptophan + 2-oxoglutarate
-
Streptomyces griseus L-glutamate + 3-indole-2-oxopropanoate
-
?
2.6.1.27 L-tryptophan + 2-oxoglutarate initial step of biosynthetic pathway of the antibiotic indolmycin Streptomyces griseus L-glutamate + 3-indole-2-oxopropanoate
-
?
2.6.1.27 L-tyrosine + 2-oxoglutarate
-
Streptomyces griseus L-glutamate + 3-(4-hydroxyphenyl)-2-oxopropanoate
-
?
2.6.1.27 additional information no activity with D-tryptophan Streptomyces griseus ?
-
?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.1.1.47 additional information
-
heating inactivates Streptomyces griseus
2.6.1.27
-
10 12 h, complete loss of activity Streptomyces griseus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.47 7.5
-
phosphate buffer Streptomyces griseus
2.1.1.47 7.5 8.5
-
Streptomyces griseus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.1.1.47 5.5
-
and below, irreversible inactivation Streptomyces griseus

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.27 pyridoxal 5'-phosphate
-
Streptomyces griseus