EC Number | General Stability | Organism |
---|---|---|
2.1.1.47 | freezing inactivates | Streptomyces griseus |
2.1.1.47 | heating inactivates | Streptomyces griseus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.1.1.47 | 1,10-phenanthroline | - |
Streptomyces griseus | |
2.1.1.47 | 2,2'-bipyridyl | - |
Streptomyces griseus | |
2.1.1.47 | iodoacetamide | weak | Streptomyces griseus | |
2.1.1.47 | iodoacetic acid | weak | Streptomyces griseus | |
2.1.1.47 | additional information | no inhibition by EDTA; not inhibitory: indolmycin, L-tryptophan, diethyldithiocarbamate, L-tryptophanylglycine, L-tryptophanamide | Streptomyces griseus | |
2.1.1.47 | N-ethylmaleimide | - |
Streptomyces griseus | |
2.1.1.47 | p-chloromercuribenzoate | strong, reversible by cysteine | Streptomyces griseus | |
2.6.1.27 | 3-Methyltryptophan | can also act as substrate | Streptomyces griseus | |
2.6.1.27 | p-chloromercuribenzoate | no inhibition | Streptomyces griseus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.1.1.47 | 0.0048 | - |
Indolepyruvate | - |
Streptomyces griseus | |
2.1.1.47 | 0.013 | - |
S-adenosyl-L-methionine | - |
Streptomyces griseus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.1.1.47 | additional information | no requirement for Mg2+, Mn2+, Ca2+ | Streptomyces griseus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.1.1.47 | 55000 | - |
gel filtration | Streptomyces griseus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.1.47 | S-adenosyl-L-methionine + indolepyruvate | Streptomyces griseus | reaction in indolmycin biosynthesis | ? | - |
? | |
2.6.1.27 | L-tryptophan + 2-oxoglutarate | Streptomyces griseus | initial step of biosynthetic pathway of the antibiotic indolmycin | L-glutamate + 3-indole-2-oxopropanoate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.1.1.47 | Streptomyces griseus | - |
- |
- |
2.6.1.27 | Streptomyces griseus | - |
strain ATCC 12648 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.1.1.47 | - |
Streptomyces griseus |
2.6.1.27 | partial, 3fold | Streptomyces griseus |
EC Number | Storage Stability | Organism |
---|---|---|
2.1.1.47 | 2°C, up to 1 month | Streptomyces griseus |
2.6.1.27 | -20°C, best stored by freezing the crude cell-free extract with 10% glycerol or by freezing the 45-60% ammonium sulfate precipitate in a phosphate buffer solution | Streptomyces griseus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.1.47 | additional information | no substrate: L-tryptophan | Streptomyces griseus | ? | - |
? | |
2.1.1.47 | additional information | no substrates are phenylpyruvate, p-hydroxyphenylpyruvate | Streptomyces griseus | ? | - |
? | |
2.1.1.47 | S-adenosyl-L-methionine + indolepyruvate | - |
Streptomyces griseus | S-adenosyl-L-homocysteine + (S)-beta-methylindolepyruvate | - |
? | |
2.1.1.47 | S-adenosyl-L-methionine + indolepyruvate | reaction in indolmycin biosynthesis | Streptomyces griseus | ? | - |
? | |
2.6.1.27 | 3-methyltryptophan + 2-oxoglutarate | can also inhibit the enzyme, stereospecific for positions 2 and 3 | Streptomyces griseus | 3-(3-methylindole)-2-oxopropanoate + L-glutamate | - |
? | |
2.6.1.27 | L-phenylalanine + 2-oxoglutarate | - |
Streptomyces griseus | L-glutamate + phenylpyruvate | - |
? | |
2.6.1.27 | L-tryptophan + 2-oxoglutarate | - |
Streptomyces griseus | L-glutamate + 3-indole-2-oxopropanoate | - |
? | |
2.6.1.27 | L-tryptophan + 2-oxoglutarate | initial step of biosynthetic pathway of the antibiotic indolmycin | Streptomyces griseus | L-glutamate + 3-indole-2-oxopropanoate | - |
? | |
2.6.1.27 | L-tyrosine + 2-oxoglutarate | - |
Streptomyces griseus | L-glutamate + 3-(4-hydroxyphenyl)-2-oxopropanoate | - |
? | |
2.6.1.27 | additional information | no activity with D-tryptophan | Streptomyces griseus | ? | - |
? |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.1.1.47 | additional information | - |
heating inactivates | Streptomyces griseus |
2.6.1.27 | - |
10 | 12 h, complete loss of activity | Streptomyces griseus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.1.1.47 | 7.5 | - |
phosphate buffer | Streptomyces griseus |
2.1.1.47 | 7.5 | 8.5 | - |
Streptomyces griseus |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
2.1.1.47 | 5.5 | - |
and below, irreversible inactivation | Streptomyces griseus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.6.1.27 | pyridoxal 5'-phosphate | - |
Streptomyces griseus |